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Literature summary for 3.2.1.21 extracted from

  • Cameron, R.G.; Manthey, J.A.; Baker, R.A.; Grohmann, K.
    Purification and characterization of a beta-glucosidase from Citrus sinensis var. Valencia fruit tissue (2001), J. Agric. Food Chem., 49, 4457-4462.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose 5 mM, approx. 50% inhibition, 100 mM, almost complete inhibition Citrus sinensis
gluconolactone 10 mM, 91% inhibition, 1 mM, 70% inhibition Citrus sinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 p-nitrophenyl-beta-D-glucopyranose 40°C, pH 5.0 Citrus sinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 x * 64000, SDS-PAGE Citrus sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arbutin + H2O Citrus sinensis 104% of activity with p-nitrophenyl-beta-D-glucopyranoside 4-hydroxyphenol + beta-D-glucose
-
 ?
cellobiose + H2O Citrus sinensis 609% of activity with p-nitrophenyl-beta-D-glucopyranoside 2 beta-D-glucose
-
 ?
gentiobiose + H2O Citrus sinensis 483% of activity with p-nitrophenyl-beta-D-glucopyranoside 2 beta-D-glucose
-
 ?
hesperetin 7-O-beta-glucopyranoside + H2O Citrus sinensis 199% of activity with p-nitrophenyl-beta-D-glucopyranoside hesperetin + beta-D-glucose
-
 ?
hesperitin-dihydrochalcon 7-O-beta-D-glucopyranoside + H2O Citrus sinensis 91% of activity with p-nitrophenyl-beta-D-glucopyranoside hesperitin-dihydrochalcon + beta-D-glucose
-
 ?
laminaribiose + H2O Citrus sinensis 519% of activity with p-nitrophenyl-beta-D-glucopyranoside 2 beta-D-glucose
-
 ?
mandelonitrile-beta-D-glucopyranoside + H2O Citrus sinensis trivial name prunasin, 309% of activity with p-nitrophenyl-beta-D-glucopyranoside mandelonitrile + beta-D-glucose
-
 ?
salicin + H2O Citrus sinensis 390% of activity with p-nitrophenyl-beta-D-glucopyranoside beta-D-glucose + ?
-
 ?
sinigrin + H2O Citrus sinensis 325% of activity with p-nitrophenyl-beta-D-glucopyranoside beta-D-glucose + ?
-
 ?
sophorose + H2O Citrus sinensis 430% of activity with p-nitrophenyl-beta-D-glucopyranoside 2 beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Citrus sinensis
-
 var. Valencia
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel, CM-Sepharose, Superdex, Toyopearl Citrus sinensis

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Citrus sinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13
-
-
 Citrus sinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O 7% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis 4-nitrophenol + D-glucose
-
 ?
arbutin + H2O 104% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis 4-hydroxyphenol + beta-D-glucose
-
 ?
cellobiose + H2O 609% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis 2 beta-D-glucose
-
 ?
gentiobiose + H2O 483% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis 2 beta-D-glucose
-
 ?
hesperetin 7-O-beta-glucopyranoside + H2O 199% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis hesperetin + beta-D-glucose
-
 ?
hesperitin-dihydrochalcon 7-O-beta-D-glucopyranoside + H2O 91% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis hesperitin-dihydrochalcon + beta-D-glucose
-
 ?
laminaribiose + H2O 519% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis 2 beta-D-glucose
-
 ?
mandelonitrile-beta-D-glucopyranoside + H2O trivial name prunasin, 309% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis mandelonitrile + beta-D-glucose
-
 ?
naringenin-7-beta-glucopyranoside + H2O 138% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis naringenin + beta-D-glucose
-
 ?
p-nitrophenyl-alpha-D-arabinofuranoside + H2O 3.7% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis p-nitrophenol + alpha-D-arabinose
-
 ?
p-nitrophenyl-alpha-D-mannopyranoside + H2O 1.1% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis p-nitrophenol + alpha-D-mannose
-
 ?
p-nitrophenyl-alpha-D-rhamnopyranoside + H2O 1.9% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis p-nitrophenol + alpha-D-rhamnose
-
 ?
p-nitrophenyl-beta-D-fucopyranoside + H2O 13.5% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis p-nitrophenol + beta-D-fucose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Citrus sinensis p-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-xylopyranoside + H2O 1.9% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis p-nitrophenol + beta-D-xylose
-
 ?
salicin + H2O 390% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis beta-D-glucose + ?
-
 ?
sinigrin + H2O 325% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis beta-D-glucose + ?
-
 ?
sophorose + H2O 430% of activity with p-nitrophenyl-beta-D-glucopyranoside Citrus sinensis 2 beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Citrus sinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Citrus sinensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 approx. 70% of maximal activity at 30°C, approx. 13% of maximal activity at 60°C Citrus sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.5
-
 Citrus sinensis

pH Range

pH Minimum pH Maximum Comment Organism
3 7 approx. 20% of maximal activity at pH 3.5, approx. 40% of maximal activity at pH 7.0 Citrus sinensis