KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.3 | - |
p-nitrophenyl-beta-D-glucopyranoside | 35°C, pH 5.0, beta-glucosidase purified from honey sac | Apis mellifera | |
12 | - |
p-nitrophenyl-beta-D-glucopyranoside | 35°C, pH 5.0, beta-glucosidase purified from ventriculus | Apis mellifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
x * 72000, SDS-PAGE | Apis mellifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apis mellifera | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Apis mellifera |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose, Superose, CM-sepharose, Mono S, hydroxyapatite, Superose 12 | Apis mellifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
honey sac | - |
Apis mellifera | - |
ventriculus | - |
Apis mellifera | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.49 | - |
beta-glucosidase purified from honey sac | Apis mellifera |
5.66 | - |
beta-glucosidase purified from ventriculus | Apis mellifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Apis mellifera | p-nitrophenol + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, SDS-PAGE | Apis mellifera |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | activity slowly increases as a function of temperature | Apis mellifera |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | no loss of activity after 20 min | Apis mellifera |
55 | - |
approx. 80% loss of activity after 20 min | Apis mellifera |
60 | - |
complete loss of activity after 20 min | Apis mellifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Apis mellifera |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | increase in activity until pH 5.5, sharp drop in activity above pH 6.0 | Apis mellifera |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 9.5 | no loss of activity after 20 min at 25°C | Apis mellifera |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Apis mellifera | isoelectric focusing, different isoenzymes probably due to different degrees of glycosylation | - |
4.5 |