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Literature summary for 3.2.1.21 extracted from
- Purification and characterization of two beta-glucosidases from the thermophilic fungus Thermoascus aurantiacus (2002), Folia Microbiol. (Praha), 47, 685-690.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | 100 mM, 69% inhibition; 100 mM, 90% inhibition | Thermoascus aurantiacus |  |
| CoCl2 | 100 mM, 54% inhibition | Thermoascus aurantiacus | |
| CuSO4 | 100 mM, 48% inhibition; 100 mM, 66% inhibition | Thermoascus aurantiacus | |
| FeCl3 | 100 mM, 59% inhibition | Thermoascus aurantiacus | |
| HgCl2 | 100 mM, 53% inhibition; 100 mM, 64% inhibition | Thermoascus aurantiacus | |
| KCl | 100 mM, 54% inhibition | Thermoascus aurantiacus | |
| MgSO4 | 100 mM, 44% inhibition | Thermoascus aurantiacus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.17 | - |
p-nitrophenyl-beta-D-glucopyranoside | 80°C, pH 4.5 | Thermoascus aurantiacus | |
| 1.38 | - |
p-nitrophenyl-beta-D-glucopyranoside | 75°C, pH 4.0 | Thermoascus aurantiacus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight stimulation | Thermoascus aurantiacus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 157000 | - |
x * 157000, SDS-PAGE | Thermoascus aurantiacus |
| 175000 | - |
x * 175000, SDS-PAGE | Thermoascus aurantiacus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoascus aurantiacus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ethanol precipitation, Sephadex G-100, Q-Sepharose | Thermoascus aurantiacus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 458.9 | - |
- |
Thermoascus aurantiacus |
| 771 | - |
- |
Thermoascus aurantiacus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Thermoascus aurantiacus | p-nitrophenol + beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 175000, SDS-PAGE | Thermoascus aurantiacus |
| ? | x * 157000, SDS-PAGE | Thermoascus aurantiacus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Gl-2 | - |
Thermoascus aurantiacus |
| Gl-3 | - |
Thermoascus aurantiacus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Thermoascus aurantiacus |
| 80 | - |
- |
Thermoascus aurantiacus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 90 | approx. 25% of maximal activity at 50°C, approx. 40% of maximal activity at 90°C | Thermoascus aurantiacus |
| 50 | 90 | approx. 30% of maximal activity at 50°C, approx. 20% of maximal activity at 90°C | Thermoascus aurantiacus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
25% loss of activity after 1 h | Thermoascus aurantiacus |
| 70 | - |
18% loss of activity after 1 h | Thermoascus aurantiacus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Thermoascus aurantiacus |
| 4.5 | - |
- |
Thermoascus aurantiacus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | approx. 65% of maximal activity at pH 3.0, approx. 10% of maximal activity at pH 6.0 | Thermoascus aurantiacus |
| 3 | 7 | approx. 80% of maximal activity at pH 3.0, approx. 18% of maximal activity at pH 6.0 | Thermoascus aurantiacus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | no loss of activity | Thermoascus aurantiacus |
| 5.5 | 8.5 | no loss of activity | Thermoascus aurantiacus |
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