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Literature summary for 3.2.1.21 extracted from

  • Vallmitjana, M.; Ferrer-Navarro, M.; Planell, R.; Abel, M.; Ausin, C.; Querol, E.; Planas, A.; Perez-Pons, J.A.
    Mechanism of the family 1 beta-glucosidase from Streptomyces sp: catalytic residues and kinetic studies (2001), Biochemistry, 40, 5975-5982.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces sp.

Protein Variants

Protein Variants Comment Organism
E178A 3500fold reduction in catalytic efficiency, E178 ist the general acid/base catalyst of Bgl3 Streptomyces sp.
E178Q 250fold reduction in catalytic efficiency Streptomyces sp.
E383A 100000fold reduction in catalytic efficiency Streptomyces sp.
E383Q 1000000fold reduction in catalytic efficiency, E383 is the catalytic nucleophile of Bgl3 Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 2,4-dinitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
0.015
-
 2,3-dinitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
0.023
-
 2-nitrophenyl beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
0.054
-
 3,5-dinitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
0.058
-
 5,11-Dimethyl-6H-pyrido[4,3-b]-carbazole 30°C, pH 6.5 Streptomyces sp.
0.064
-
 3,4-dinitrophenyl beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
0.1
-
 p-nitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
0.14
-
 p-nitrophenyl-beta-D-fucopyranoside 50°C, pH 6.5 Streptomyces sp.
0.15
-
 p-nitrophenyl-beta-D-glucopyranoside 50°C, pH 6.5 Streptomyces sp.
0.24
-
 3-nitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
0.47
-
 4-bromophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
2.3
-
 phenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
4.1
-
 cellobiose 50°C, pH 6.5 Streptomyces sp.
7.3
-
 p-nitrophenyl-beta-D-galactopyranoside 50°C, pH 6.5 Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + H2O Streptomyces sp.
-
 2 beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Bgl3 Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dinitrophenyl-beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 2,3-dinitrophenol + beta-D-glucose
-
 ?
2,4-dinitrophenyl-beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 2,4-dinitrophenol + beta-D-glucose
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 2-nitrophenol + beta-D-glucopyranose
-
 ?
3,4-dinitrophenyl beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 3,4-dinitrophenol + beta-D-glucose
-
 ?
3,4-dinitrophenyl beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 3,4-dinitrophenol + beta-D-glucopyranose
-
 ?
3,5-dinitrophenyl beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 3,5-dinitrophenol + D-glucose
-
 ?
3-nitrophenyl beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 3-nitrophenol + D-glucose
-
 ?
4-bromophenyl-beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 4-bromophenol + beta-D-glucose
-
 ?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 4-methylumbelliferone + beta-D-glucose
-
 ?
5,11-dimethyl-6H-pyrido[4,3-b]-carbazole + H2O
-
 Streptomyces sp. ?
-
 ?
cellobiose + H2O
-
 Streptomyces sp. 2 beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-fucopyranoside + H2O
-
 Streptomyces sp. p-nitrophenol + beta-D-fucose
-
 ?
p-nitrophenyl-beta-D-galactopyranoside + H2O
-
 Streptomyces sp. p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Streptomyces sp. p-nitrophenol + beta-D-glucose
-
 ?
phenyl-beta-D-glucpyranoside + H2O
-
 Streptomyces sp. phenol + beta-D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
Bgl3
-
 Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55
-
 Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.66
-
 phenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.32
-
 4-bromophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
7.72
-
 2-nitrophenyl beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
8.14
-
 p-nitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
11.7
-
 4-methylumbelliferyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
11.7
-
 2,3-dinitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
15.8
-
 3-nitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
18.2
-
 3,4-dinitrophenyl beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
20.5
-
 3,5-dinitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
28.4
-
 p-nitrophenyl-beta-D-glucopyranoside 50°C, pH 6.5 Streptomyces sp.
30.4
-
 2,4-dinitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
35.6
-
 cellobiose 50°C, pH 6.5 Streptomyces sp.
37.1
-
 p-nitrophenyl-beta-D-fucopyranoside 50°C, pH 6.5 Streptomyces sp.
118
-
 p-nitrophenyl-beta-D-galactopyranoside 50°C, pH 6.5 Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 approx. 505 of maximal activity at pH 5.5, approx. 25% of maximal activity at pH 7.0 Streptomyces sp.