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Literature summary for 3.2.1.21 extracted from

  • Berrin, J.G.; Czjzek, M.; Kroon, P.A.; McLauchlan, W.R.; Puigserver, A.; Williamson, G.; Juge, N.
    Substrate (aglycone) specificity of human cytosolic beta-glucosidase (2003), Biochem. J., 373, 41-48.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Homo sapiens

Protein Variants

Protein Variants Comment Organism
F225S 0.65% of wild-type specific activity with p-nitrophenyl-beta-D-glucopyranoside Homo sapiens
V168Y 12% of wild-type specific activity with p-nitrophenyl-beta-D-glucopyranoside Homo sapiens
Y308A 0.3% of wild-type specific activity with p-nitrophenyl-beta-D-glucopyranoside Homo sapiens
Y308F 6% of wild-type specific activity with p-nitrophenyl-beta-D-glucopyranoside Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
 p-nitrophenyl-beta-D-fucopyranoside 50°C, pH 6.5 Homo sapiens
0.57
-
 p-nitrophenyl-beta-D-arabinopyranoside 50°C, pH 6.5 Homo sapiens
1.76
-
 p-nitrophenyl-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.14
-
 p-nitrophenyl-beta-D-galactopyranoside 50°C, pH 6.5 Homo sapiens
10
-
 luteolin-4'-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
31.8
-
 quercetin-4'-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
42.2
-
 quercetin-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
50
-
 luteolin-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
118
-
 daidzein 7-O-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
253
-
 eriodictyol-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
432
-
 naringenin-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 1 * 53000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
apigenin-7-beta-glucopyranoside + H2O Homo sapiens
-
 apigenin + beta-D-glucose
-
 ?
daidzein 7-O-beta-D-glucopyranoside + H2O Homo sapiens
-
 daidzein + beta-D-glucose
-
 ?
eriodicytol-7-beta-D-glucopyranoside + H2O Homo sapiens
-
 eriodicytol + beta-D-glucose
-
 ?
luteolin-4'-beta-glucopyranoside + H2O Homo sapiens
-
 luteolin + beta-D-glucose
-
 ?
luteolin-7-beta-glucopyranoside + H2O Homo sapiens
-
 luteolin + beta-D-glucose
-
 ?
naringenin-7-beta-glucopyranoside + H2O Homo sapiens
-
 naringenin + beta-D-glucose
-
 ?
quercetin 4'-O-beta-D-glucopyranoside + H2O Homo sapiens
-
 quercetin + beta-D-glucose
-
 ?
quercetin 7-O-beta-D-glucopyranoside + H2O Homo sapiens
-
 quercetin + beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant hCBG Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
spleen
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apigenin-7-beta-glucopyranoside + H2O
-
 Homo sapiens apigenin + beta-D-glucose
-
 ?
daidzein 7-O-beta-D-glucopyranoside + H2O
-
 Homo sapiens daidzein + beta-D-glucose
-
 ?
eriodicytol-7-beta-D-glucopyranoside + H2O
-
 Homo sapiens eriodicytol + beta-D-glucose
-
 ?
luteolin-4'-beta-glucopyranoside + H2O
-
 Homo sapiens luteolin + beta-D-glucose
-
 ?
luteolin-7-beta-glucopyranoside + H2O
-
 Homo sapiens luteolin + beta-D-glucose
-
 ?
naringenin-7-beta-glucopyranoside + H2O
-
 Homo sapiens naringenin + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-arabinopyranoside + H2O
-
 Homo sapiens p-nitrophenol + beta-D-arabinose
-
 ?
p-nitrophenyl-beta-D-fucopyranoside + H2O
-
 Homo sapiens p-nitrophenol + beta-D-fucose
-
 ?
p-nitrophenyl-beta-D-galactopyranoside + H2O
-
 Homo sapiens p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Homo sapiens p-nitrophenol + beta-D-glucose
-
 ?
quercetin 4'-O-beta-D-glucopyranoside + H2O
-
 Homo sapiens quercetin + beta-D-glucose
-
 ?
quercetin 7-O-beta-D-glucopyranoside + H2O
-
 Homo sapiens quercetin + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 53000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
hCBG
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 20% loss of activity after 24 h Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.69
-
 quercetin-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
1.08
-
 quercetin-4'-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
1.17
-
 luteolin-4'-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
1.26
-
 eriodictyol-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
2.6
-
 naringenin-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.05
-
 luteolin-7-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
3.55
-
 daidzein 7-O-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
6
-
 p-nitrophenyl-beta-D-arabinopyranoside 50°C, pH 6.5 Homo sapiens
10.7
-
 p-nitrophenyl-beta-D-fucopyranoside 50°C, pH 6.5 Homo sapiens
12.1
-
 p-nitrophenyl-beta-D-glucopyranoside 50°C, pH 6.5 Homo sapiens
17.6
-
 p-nitrophenyl-beta-D-galactopyranoside 50°C, pH 6.5 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens
-
-
 4.9