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Literature summary for 3.2.1.21 extracted from

  • Calsavara, L.P.; De Moraes, F.F.; Zanin, G.M.
    Comparison of catalytic properties of free and immobilized cellobiase novozym 188 (2001), Appl. Biochem. Biotechnol., 91-93, 615-626.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + H2O Aspergillus niger
-
2 beta-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
novozym 188
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.5
-
50°C, pH 4.8 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + H2O
-
Aspergillus niger 2 beta-D-glucose
-
?

Synonyms

Synonyms Comment Organism
cellobiase
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 75 approx. 20% of maximal activity at 40°C and pH 4.5, approx. 15% of maximal activity at 70°C and pH 4.5 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 55 no loss of activity after 240 min Aspergillus niger
60
-
approx. 15% loss of activity after 240 min Aspergillus niger
65
-
approx. 70% loss of activity after 240 min Aspergillus niger
70
-
complete loss of activity after 90 min Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
3 7 approx. 30% of maximal activity at pH 3.0 and 65°C, approx. 5% of maximal activity at pH 6.0 and 65°C Aspergillus niger