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Literature summary for 3.2.1.21 extracted from

  • Yoshioka, H.; Hayashida, S.
    Production and purification of thermostable beta-glucosidase from Mucor miehei YH-10 (1980), Agric. Biol. Chem., 44, 2817-2824.
No PubMed abstract available
Search for more literature for 3.2.1.21

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Rhizomucor miehei
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Rhizomucor miehei cellobiose beta-D-glucose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizomucor miehei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques, 2 isoforms Rhizomucor miehei

Source Tissue

Source Tissue Comment Organism Textmining
cell culture maximal enzyme synthesis, if cells grown in wheat bran medium Rhizomucor miehei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Rhizomucor miehei beta-D-glucose + D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Rhizomucor miehei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 isoform G-a, stable for 5 min Rhizomucor miehei
80
-
 isoform G-b, completely inactivated after 5 min Rhizomucor miehei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Rhizomucor miehei

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 10 stable within for 24 h at 4°C Rhizomucor miehei