Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Ruminococcus albus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
gel filtration | Ruminococcus albus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Ruminococcus albus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Ruminococcus albus | activity 60fold less than for 4-nitrophenyl-beta-D-glucopyranoside | beta-D-glucose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminococcus albus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, recombinant enzyme | Ruminococcus albus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | - |
Ruminococcus albus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-cellobioside + H2O | - |
Ruminococcus albus | 4-methylumbelliferone + beta-D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-glucoside + H2O | - |
Ruminococcus albus | 4-methylumbelliferone + beta-D-glucose | - |
? | |
4-nitrophenyl beta-cellobioside + 2 H2O | - |
Ruminococcus albus | 4-nitrophenol + 2 beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Ruminococcus albus | 4-nitrophenol + D-glucopyranose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Ruminococcus albus | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | activity 60fold less than for 4-nitrophenyl-beta-D-glucopyranoside | Ruminococcus albus | beta-D-glucose + D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Ruminococcus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Ruminococcus albus |