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Literature summary for 3.2.1.21 extracted from

  • Sadana, J.C.; Patil, R.V.; Shewale, J.G.
    beta-D-Glucosidases from Sclerotium rolfsii (1988), Methods Enzymol., 160, 424-431.
No PubMed abstract available
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose Ki: 0.55 mM Athelia rolfsii
D-glucono-1,5-lactone Ki: 0.01 mM Athelia rolfsii
nojirimycin bisulfite Ki: 0.001 mM Athelia rolfsii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.79
-
 4-nitrophenyl-beta-D-glucopyranoside beta-glucosidase 4 Athelia rolfsii
0.89
-
 4-nitrophenyl-beta-D-glucopyranoside beta-glucosidase 3 Athelia rolfsii
1.07
-
 4-nitrophenyl-beta-D-glucopyranoside beta-glucosidase 1 Athelia rolfsii
1.38
-
 4-nitrophenyl-beta-D-glucopyranoside beta-glucosidase 2 Athelia rolfsii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 beta-glucosidases 1 and 2, gel filtration Athelia rolfsii
92000
-
 beta-glucosidase 4, gel filtration Athelia rolfsii
107000
-
 beta-glucosidase 3, gel filtration Athelia rolfsii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Athelia rolfsii sophorose beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Athelia rolfsii laminaribiose beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Athelia rolfsii not restricted to the beta-D-1,4-linkage beta-D-glucose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Athelia rolfsii cellobiose beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Athelia rolfsii gentiobiose beta-D-glucose + D-glucose
-
 ?
cellohexaose + 5 H2O Athelia rolfsii
-
 6 D-glucose
-
 ?
cellopentaose + 4 H2O Athelia rolfsii preferred substrate 5 D-glucose
-
 ?
cellotetraose + 3 H2O Athelia rolfsii
-
 4 D-glucose
-
 ?
cellotriose + 2 H2O Athelia rolfsii
-
 3 D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Athelia rolfsii
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Athelia rolfsii

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 4 different forms, chromatography steps Athelia rolfsii

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Athelia rolfsii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25 57 4 different forms Athelia rolfsii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Athelia rolfsii 2-nitrophenol + D-glucopyranose
-
 ?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Athelia rolfsii beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Athelia rolfsii beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O not restricted to the beta-D-1,4-linkage Athelia rolfsii beta-D-glucose + D-glucose
-
 ?
4-nitrophenyl beta-cellobioside + 2 H2O
-
 Athelia rolfsii 4-nitrophenol + 2 beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Athelia rolfsii 4-nitrophenol + D-glucopyranose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Athelia rolfsii beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Athelia rolfsii beta-D-glucose + D-glucose
-
 ?
cellohexaose + 5 H2O
-
 Athelia rolfsii 6 D-glucose
-
 ?
cellopentaose + 4 H2O preferred substrate Athelia rolfsii 5 D-glucose
-
 ?
cellotetraose + 3 H2O
-
 Athelia rolfsii 4 D-glucose
-
 ?
cellotriose + 2 H2O
-
 Athelia rolfsii 3 D-glucose
-
 ?
methyl-beta-D-glucoside + H2O
-
 Athelia rolfsii beta-D-glucose + methanol
-
 ?
phenyl-beta-D-glucoside + H2O
-
 Athelia rolfsii phenol + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 90000-107000, SDS-PAGE, 4 isoforms Athelia rolfsii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
 for 4 different forms Athelia rolfsii