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Literature summary for 3.2.1.21 extracted from
- Purification of the beta-glucosidase from Sclerotinia sclerotiorum (1988), Biochim. Biophys. Acta, 967, 82-86.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Sclerotinia sclerotiorum |  |
| 1.23 | - |
cellobiose | - |
Sclerotinia sclerotiorum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Sclerotinia sclerotiorum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 240000 | - |
gel filtration | Sclerotinia sclerotiorum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Sclerotinia sclerotiorum | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Sclerotinia sclerotiorum | promoting action of 1,4-beta-cellobiohydrolase | beta-D-glucose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sclerotinia sclerotiorum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Sclerotinia sclerotiorum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Sclerotinia sclerotiorum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Sclerotinia sclerotiorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Sclerotinia sclerotiorum | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Sclerotinia sclerotiorum | 4-nitrophenol + beta-D-xylose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Sclerotinia sclerotiorum | beta-D-glucose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | promoting action of 1,4-beta-cellobiohydrolase | Sclerotinia sclerotiorum | beta-D-glucose + D-glucose | - |
? | |
| cellobiose + H2O | - |
Sclerotinia sclerotiorum | 2 beta-D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Sclerotinia sclerotiorum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
rapidly inactivated above | Sclerotinia sclerotiorum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Sclerotinia sclerotiorum |
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