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Literature summary for 3.2.1.21 extracted from

  • Martinez, M.J.; Vazquez, C.; Guillen, F.; Reyes, F.
    beta-Glucosidase from the cellulolytic system of Alternaria alternata autolyzed cultures (1988), FEMS Microbiol. Lett., 55, 263-268.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
D-glucono-1,5-lactone 45% inhibition at 10 mM Alternaria alternata
Hg2+ strong inhibition at 5 mM Alternaria alternata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 cellobiose
-
 Alternaria alternata
1.1
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Alternaria alternata
5
-
 Salicin
-
 Alternaria alternata
16.6
-
 4-nitrophenyl-beta-D-galactoside
-
 Alternaria alternata
20
-
 4-nitrophenyl-alpha-D-glucoside
-
 Alternaria alternata
50
-
 Laminarin
-
 Alternaria alternata

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Alternaria alternata
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 1 * 80000 + 1 * 60000, SDS-PAGE Alternaria alternata
80000
-
 1 * 80000 + 1 * 60000, SDS-PAGE Alternaria alternata
160000
-
 gel filtration Alternaria alternata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Alternaria alternata cellobiose beta-D-glucose + D-glucose
-
 ?
laminarin + H2O Alternaria alternata involvement in autolysis beta-D-glucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Alternaria alternata
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content: 11% galactose, 4% mannose, 1% glucose Alternaria alternata

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Alternaria alternata

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Alternaria alternata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.3
-
-
 Alternaria alternata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Alternaria alternata 2-hydroxymethylphenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O
-
 Alternaria alternata 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Alternaria alternata 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl-alpha-D-glucoside
-
 Alternaria alternata 4-nitrophenol + alpha-D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Alternaria alternata beta-D-glucose + D-glucose
-
 ?
cellobiose + H2O
-
 Alternaria alternata 2 beta-D-glucose
-
 ?
laminarin + H2O involvement in autolysis Alternaria alternata beta-D-glucose + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 80000 + 1 * 60000, SDS-PAGE Alternaria alternata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Alternaria alternata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 60 stable for 1 h Alternaria alternata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Alternaria alternata

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 stable within Alternaria alternata