KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
4-nitrophenyl-beta-D-glucopyranoside | non-immobilized soluble enzyme | Thermothelomyces heterothallicus | |
5 | - |
4-nitrophenyl-beta-D-glucopyranoside | immobilized enzyme to polyacrylamide gel | Thermothelomyces heterothallicus | |
5.2 | - |
4-nitrophenyl-beta-D-glucopyranoside | immobilized enzyme to CNBr-Sepharose | Thermothelomyces heterothallicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermothelomyces heterothallicus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces heterothallicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Thermothelomyces heterothallicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Thermothelomyces heterothallicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermothelomyces heterothallicus | 4-nitrophenol + D-glucopyranose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
non-immobilized enzyme preparation | Thermothelomyces heterothallicus |
70 | - |
immobilized enzyme to CNBr-Sepharose | Thermothelomyces heterothallicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for 1 h, immobilized enzyme shows better stability | Thermothelomyces heterothallicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
for non-immobilized and immobilized enzyme preparations | Thermothelomyces heterothallicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6 | stable within, soluble and immobilized enzyme preparations | Thermothelomyces heterothallicus |