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Literature summary for 3.2.1.21 extracted from

  • Roy, S.K.; Raha, S.K.; Dey, S.K.; Chakrabarty, S.L.
    Immobilization of beta-glucosidase from Myceliophthora thermophila D-14 (1989), Enzyme Microb. Technol., 11, 431-435.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5
-
4-nitrophenyl-beta-D-glucopyranoside non-immobilized soluble enzyme Thermothelomyces heterothallicus
5
-
4-nitrophenyl-beta-D-glucopyranoside immobilized enzyme to polyacrylamide gel Thermothelomyces heterothallicus
5.2
-
4-nitrophenyl-beta-D-glucopyranoside immobilized enzyme to CNBr-Sepharose Thermothelomyces heterothallicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermothelomyces heterothallicus
-
-

Organism

Organism UniProt Comment Textmining
Thermothelomyces heterothallicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Thermothelomyces heterothallicus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Thermothelomyces heterothallicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermothelomyces heterothallicus 4-nitrophenol + D-glucopyranose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
non-immobilized enzyme preparation Thermothelomyces heterothallicus
70
-
immobilized enzyme to CNBr-Sepharose Thermothelomyces heterothallicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable for 1 h, immobilized enzyme shows better stability Thermothelomyces heterothallicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
for non-immobilized and immobilized enzyme preparations Thermothelomyces heterothallicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 6 stable within, soluble and immobilized enzyme preparations Thermothelomyces heterothallicus