Literature summary for 3.2.1.21 extracted from

Ong, E.; Gilkes, N.R.; Miller, R.C.; Warren, R.A.J.; Kilburn, D.G.
Enzyme immobilization using a cellulose-binding domain: properties of a beta-glucosidase fusion protein (1991), Enzyme Microb. Technol., 13, 59-65.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Agrobacterium sp.

General Stability

General Stability	Organism
cellulose-binding domain-beta-glucosidase fusion protein: in solution stable for 24 h at 25°C, pH 7.0, if immobilized on cellulose: stable up to 7 weeks at 4°C or 37°C	Agrobacterium sp.

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium sp.	-	fused enzyme to a cellulose-binding domain from Cex-exoglucanase	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity, as fusion protein by affinity chromatography	Agrobacterium sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Agrobacterium sp.	4-nitrophenol + D-glucopyranose	-	?

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	9	rapid inactivation below and above for cellulose-immobilized Cex-cellulose-binding domain fused to beta-glucosidase	Agrobacterium sp.

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Release 2023.1 (January 2023)