Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-D-glucose | cloned enzyme is activated 2-3fold at 0.1-0.3 M | Thermobispora bispora |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermobispora bispora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52280 | - |
non-denaturating gradient PAGE | Thermobispora bispora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermobispora bispora | cellobiose | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermobispora bispora | cloned enzyme show greater activity against cellobiose than against aryl-beta-D-glucosides | beta-D-glucose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobispora bispora | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, recombinant enzyme | Thermobispora bispora |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | genomic library | Thermobispora bispora | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-glucoside + H2O | - |
Thermobispora bispora | 4-methylumbelliferone + beta-D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Thermobispora bispora | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cloned enzyme show greater activity against cellobiose than against aryl-beta-D-glucosides | Thermobispora bispora | beta-D-glucose + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BglB | - |
Thermobispora bispora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermobispora bispora |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
retaining 70% of its activity after 48 h | Thermobispora bispora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Thermobispora bispora |