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Literature summary for 3.2.1.21 extracted from

  • Lin, J.; Pillay, B.; Singh, S.
    Purification and biochemical characteristics of beta-D-glucosidase from a thermophilic fungus, Thermomyces lanuginosus - SSBP (1999), Biotechnol. Appl. Biochem., 30, 81-87.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
L-cysteine 50% increase of activity Thermomyces lanuginosus

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose competitive inhibition, Ki: 0.55 mM Thermomyces lanuginosus
cellobiose competitive inhibition, Ki: 0.52 mM Thermomyces lanuginosus
Hg2+ complete inhibition at 2 mM Thermomyces lanuginosus
N-bromosuccinimide complete inhibition at 2 mM Thermomyces lanuginosus
Salicin competitive inhibition, Ki: 0.81 mM Thermomyces lanuginosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
4-nitrophenyl-beta-D-glucopyranoside
-
Thermomyces lanuginosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermomyces lanuginosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
101000
-
2 * 101000, SDS-PAGE Thermomyces lanuginosus
200000
-
gel filtration Thermomyces lanuginosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-alpha-D-glucopyranosyl-D-glucose Thermomyces lanuginosus maltose alpha-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermomyces lanuginosus cellobiose beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Thermomyces lanuginosus low activity beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Thermomyces lanuginosus gentiobiose beta-D-glucose + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Thermomyces lanuginosus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Thermomyces lanuginosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.65
-
-
Thermomyces lanuginosus

Storage Stability

Storage Stability Organism
4°C, 20 mM Tris-HCl, pH 7.1, 4 months Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity Thermomyces lanuginosus 2-hydroxymethylphenol + beta-D-glucose
-
?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Thermomyces lanuginosus 2-hydroxymethylphenol + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O best substrate Thermomyces lanuginosus 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O low activity Thermomyces lanuginosus 4-nitrophenol + beta-D-xylose
-
?
4-nitrophenyl-alpha-D-glucoside less, but significant hydrolysis Thermomyces lanuginosus 4-nitrophenol + alpha-D-glucose
-
?
4-O-alpha-D-glucopyranosyl-D-glucose maltose Thermomyces lanuginosus alpha-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Thermomyces lanuginosus beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Thermomyces lanuginosus beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Thermomyces lanuginosus beta-D-glucose + D-glucose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 101000, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable within 30 min Thermomyces lanuginosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
44.4
-
4-nitrophenyl-beta-D-glucopyranoside
-
Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Thermomyces lanuginosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
rapid decrease of activity above Thermomyces lanuginosus