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Literature summary for 3.2.1.209 extracted from

  • Liebminger, E.; Huettner, S.; Vavra, U.; Fischl, R.; Schoberer, J.; Grass, J.; Blaukopf, C.; Seifert, G.J.; Altmann, F.; Mach, L.; Strasser, R.
    Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana (2009), Plant Cell, 21, 3850-3867 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.209

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Nicotiana benthamiana leaf epidermal cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin
-
 Arabidopsis thaliana
EDTA
-
 Arabidopsis thaliana
kifunensine
-
 Arabidopsis thaliana
additional information not inhibitory: swainsonine Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Arabidopsis thaliana 5783
-
Golgi apparatus isoform MNS3 probably resides in the cis-Golgi. the C-terminal region of MNS3 is sufficient for proper subcellular targeting Arabidopsis thaliana 5794
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q93Y37
-
-
Arabidopsis thaliana Q93Y37 isoform MNS3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + H2O
-
 Arabidopsis thaliana Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
-
 ?
methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
-
 Arabidopsis thaliana ? + D-mannose
-
 ?
[alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc]-N-Asn-[protein] + H2O
-
 Arabidopsis thaliana [alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc]-N-Asn-[protein] + D-mannopyranose
-
 ?

Synonyms

Synonyms Comment Organism
MNS3
-
 Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0003
-
 pH 6.0, 37°C, substrate methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside Arabidopsis thaliana kifunensine
0.035
-
 pH 6.0, 37°C, substrate methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside Arabidopsis thaliana 1-deoxymannojirimycin

General Information

General Information Comment Organism
physiological function in the MNS3 single mutant, formation of aberrant N-glycans is observed. A triple mutant lacking mannosidases MNS1, MNS2, both EC 3.2.1.209, and MNS3 reveals the almost exclusive presence of Man9GlcNAc2 glycans. The MNS triple mutants display short, radially swollen roots and altered cell walls. Pharmacological inhibition of class I alpha-mannosidases in wild-type seedlings results in a similar root phenotype Arabidopsis thaliana
physiological function mutation results in the formation of aberrant N-glycans in the Mns3 single mutant. In the Mns1/Mns2/Mns3 triple mutant, Man(9)GlcNAc(2) glycans are almost exclusively present. The Mns triple mutants display short, radially swollen roots and altered cell walls Arabidopsis thaliana