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Literature summary for 3.2.1.207 extracted from
- Glucosidase II beta subunit modulates N-glycan trimming in fission yeasts and mammals (2009), Mol. Biol. Cell, 20, 3974-3984.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the interaction of the mannose 6-phosphate receptor homologous domain present in GIIbeta with mannoses in the B and/or C arms of the glycans mediates glycan hydrolysis enhancement | Rattus norvegicus | |
| additional information | the interaction of the mannose 6-phosphate receptor homologous domain present in GIIbeta with mannoses in the B and/or C arms of the glycans mediates glycan hydrolysis enhancement | Schizosaccharomyces pombe |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of alpha- and beta-subunits and expression in Escherichia coli strains DH5alpha and JA226 | Schizosaccharomyces pombe |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E457Q | site-directed mutagenesis | Schizosaccharomyces pombe |
| additional information | construction of alpha or beta-subunit deletion mutant strains, disruption of subunit GIIalpha leads to complete loss of enzyme activity, while in the absence of GIIbeta, the catalytic subunit GIIalpha of Schizosaccharomyces pombe folds to an active conformation able to hydrolyze 4-nitrophenyl alpha-D-glucopyranoside, phenotypes, overview | Schizosaccharomyces pombe |
| Q408E | site-directed mutagenesis | Schizosaccharomyces pombe |
| R438K | site-directed mutagenesis | Schizosaccharomyces pombe |
| Y463F | site-directed mutagenesis | Schizosaccharomyces pombe |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum membrane | - |
Rattus norvegicus | 5789 | - |
| endoplasmic reticulum membrane | - |
Schizosaccharomyces pombe | 5789 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glc2Man9GlcNAc2 + H2O | Rattus norvegicus | - |
GlcMan9GlcNAc2 + D-glucopyranose | - |
? |  |
| Glc2Man9GlcNAc2 + H2O | Schizosaccharomyces pombe | - |
GlcMan9GlcNAc2 + D-glucopyranose | - |
? | |
| GlcMan9GlcNAc2 + H2O | Rattus norvegicus | - |
Man9GlcNAc2 + D-glucopyranose | - |
? | |
| GlcMan9GlcNAc2 + H2O | Schizosaccharomyces pombe | - |
Man9GlcNAc2 + D-glucopyranose | - |
? | |
| additional information | Rattus norvegicus | the enzyme heterodimer is required to efficiently deglucosylate the physiological substrates G2M9 and G1M9 | ? | - |
? | |
| additional information | Schizosaccharomyces pombe | the enzyme heterodimer is required to efficiently deglucosylate the physiological substrates G2M9 and G1M9 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
| Schizosaccharomyces pombe | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | the GIIbeta subunit is not required for GIIalpha activity toward the substrate | Rattus norvegicus | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | the GIIbeta subunit is not required for GIIalpha activity toward the substrate | Schizosaccharomyces pombe | 4-nitrophenol + D-glucopyranose | - |
? | |
| Glc2Man9GlcNAc2 + H2O | - |
Rattus norvegicus | GlcMan9GlcNAc2 + D-glucopyranose | - |
? | |
| Glc2Man9GlcNAc2 + H2O | - |
Schizosaccharomyces pombe | GlcMan9GlcNAc2 + D-glucopyranose | - |
? | |
| Glc2Man9GlcNAc2 + H2O | i.e. G1M9, alpha-mannosidase-treated glycan from jack bean, structure, overview | Rattus norvegicus | GlcMan9GlcNAc2 + D-glucopyranose | - |
? | |
| Glc2Man9GlcNAc2 + H2O | i.e. G1M9, alpha-mannosidase-treated glycan from jack bean, structure, overview | Schizosaccharomyces pombe | GlcMan9GlcNAc2 + D-glucopyranose | - |
? | |
| GlcMan9GlcNAc2 + H2O | - |
Rattus norvegicus | Man9GlcNAc2 + D-glucopyranose | - |
? | |
| GlcMan9GlcNAc2 + H2O | - |
Schizosaccharomyces pombe | Man9GlcNAc2 + D-glucopyranose | - |
? | |
| GlcMan9GlcNAc2 + H2O | i.e. G2M9, alpha-mannosidase-treated glycan from jack bean, structure, overview | Rattus norvegicus | Man9GlcNAc2 + D-glucopyranose | - |
? | |
| GlcMan9GlcNAc2 + H2O | i.e. G2M9, alpha-mannosidase-treated glycan from jack bean, structure, overview | Schizosaccharomyces pombe | Man9GlcNAc2 + D-glucopyranose | - |
? | |
| additional information | the enzyme heterodimer is required to efficiently deglucosylate the physiological substrates G2M9 and G1M9 | Rattus norvegicus | ? | - |
? | |
| additional information | the enzyme heterodimer is required to efficiently deglucosylate the physiological substrates G2M9 and G1M9 | Schizosaccharomyces pombe | ? | - |
? | |
| additional information | the interaction of the mannose 6-phosphate receptor homologous domain present in GIIbeta with mannoses in the B and/or C arms of the glycans mediates glycan hydrolysis enhancement | Rattus norvegicus | ? | - |
? | |
| additional information | the interaction of the mannose 6-phosphate receptor homologous domain present in GIIbeta with mannoses in the B and/or C arms of the glycans mediates glycan hydrolysis enhancement | Schizosaccharomyces pombe | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | the enzyme is a heterodimer whose alpha-subunit GIIalpha bears the glycosyl hydrolase active site, whereas its beta-subunit GIIbeta is involved in GIIbeta endoplasmic reticulum retention and folding, but does not efficiently deglucosylate the physiological substrates Glc2Man9GlcNAc2 and Glc1Man9GlcNAc2 | Rattus norvegicus |
| heterodimer | the enzyme is a heterodimer whose alpha-subunit GIIalpha bears the glycosyl hydrolase active site, whereas its beta-subunit GIIbeta is involved in GIIbeta endoplasmic reticulum retention and folding, but does not efficiently deglucosylate the physiological substrates Glc2Man9GlcNAc2 and GlcMan9GlcNAc2. GIIbeta is required for an efficient in vitro glucose trimming from G2M9 and G1M9, and processing of both middle and innermost glucoses | Schizosaccharomyces pombe |
| More | the interaction of the mannose 6-phosphate receptor homologous domain present in GIIbeta with mannoses in the B and/or C arms of the glycans mediates glycan hydrolysis enhancement | Rattus norvegicus |
| More | the interaction of the mannose 6-phosphate receptor homologous domain present in GIIbeta with mannoses in the B and/or C arms of the glycans mediates glycan hydrolysis enhancement | Schizosaccharomyces pombe |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucosidase II | - |
Rattus norvegicus |
| glucosidase II | - |
Schizosaccharomyces pombe |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Schizosaccharomyces pombe |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Rattus norvegicus |
| 7.2 | - |
assay at | Schizosaccharomyces pombe |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | glucosidase II plays a key role in glycoprotein biogenesis in the endoplasmic reticulum. It is responsible for the sequential removal of the two innermost glucose residues from the glycan, Glc3Man9GlcNAc2, transferred to Asn residues in proteins. The enzyme participates in the calnexin/calreticulin cycle, it removes the single glucose unit added to folding intermediates and misfolded glycoproteins by the UDP-Glc:glycoprotein glucosyltransferase | Rattus norvegicus |
| physiological function | glucosidase II plays a key role in glycoprotein biogenesis in the endoplasmic reticulum. It is responsible for the sequential removal of the two innermost glucose residues from the glycan, Glc3Man9GlcNAc2, transferred to Asn residues in proteins. The enzyme participates in the calnexin/calreticulin cycle, it removes the single glucose unit added to folding intermediates and misfolded glycoproteins by the UDP-Glc:glycoprotein glucosyltransferase | Schizosaccharomyces pombe |
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Release 2023.1 (January 2023)
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