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Literature summary for 3.2.1.207 extracted from

  • Watanabe, T.; Totani, K.; Matsuo, I.; Maruyama, J.; Kitamoto, K.; Ito, Y.
    Genetic analysis of glucosidase II beta-subunit in trimming of high-mannose-type glycans (2009), Glycobiology, 19, 834-840.
    View publication on PubMed
Search for more literature for 3.2.1.207

Cloned(Commentary)

Cloned (Comment) Organism
genes Aogls2alpha and/or Aogls2beta encdoing the alpha- und beta-subunit, cloning and expression in Escherichia coli strain DH5alpha Aspergillus oryzae

Protein Variants

Protein Variants Comment Organism
additional information construction of strains of gene disruptants lacking either the glucosidase II alpha- or beta-subunit. The mutant lacking the beta-subunit is inactive with both Glc2Man9GlcNAc2 and Glc1Man9GlcNAc2, but activity can be restored by adding the beta-subunit fraction, overview Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
deoxynojirimycin an alpha-glucosidase-specific inhibitor Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
 Aspergillus oryzae 5789
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aspergillus oryzae glucosidase II is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from N-linked oligosaccharides in the endoplasmic reticulum ?
-
 ?
additional information Aspergillus oryzae RIB 40 glucosidase II is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from N-linked oligosaccharides in the endoplasmic reticulum ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-
Aspergillus oryzae RIB 40
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Aspergillus oryzae 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Aspergillus oryzae RIB 40 4-nitrophenol + D-glucopyranose
-
 ?
Glc2Man9GlcNAc2 + H2O synthetic methotrexate-coupled glycan substrate, G1M9-MTX Aspergillus oryzae GlcMan9GlcNAc2 + D-glucopyranose
-
 ?
Glc2Man9GlcNAc2 + H2O synthetic methotrexate-coupled glycan substrate, G1M9-MTX Aspergillus oryzae RIB 40 GlcMan9GlcNAc2 + D-glucopyranose
-
 ?
GlcMan9GlcNAc2 + H2O synthetic methotrexate-coupled glycan substrate, G2M9-MTX Aspergillus oryzae Man9GlcNAc2 + D-glucopyranose
-
 ?
GlcMan9GlcNAc2 + H2O synthetic methotrexate-coupled glycan substrate, G2M9-MTX Aspergillus oryzae RIB 40 Man9GlcNAc2 + D-glucopyranose
-
 ?
additional information glucosidase II is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from N-linked oligosaccharides in the endoplasmic reticulum Aspergillus oryzae ?
-
 ?
additional information the mutant lacking the beta-subunit is inactive with both Glc2Man9GlcNAc2 and GlcMan9GlcNAc2 Aspergillus oryzae ?
-
 ?
additional information glucosidase II is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from N-linked oligosaccharides in the endoplasmic reticulum Aspergillus oryzae RIB 40 ?
-
 ?
additional information the mutant lacking the beta-subunit is inactive with both Glc2Man9GlcNAc2 and GlcMan9GlcNAc2 Aspergillus oryzae RIB 40 ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer the enzyme is a heterodimer whose alpha-subunit contains a glycosidase active site, while the beta-subunit confers the substrate specificity toward di- and monoglucosylated glycans on the glucose-trimming activity of the alpha-subunit Aspergillus oryzae

Synonyms

Synonyms Comment Organism
glucosidase II
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Aspergillus oryzae