Literature summary for 3.2.1.207 extracted from

Soussillane, P.; D'Alessio, C.; Paccalet, T.; Fitchette, A.-C.; Parodi, A.J.; Williamson, R.; Plasson, C.; Faye, L.; Gomord, V.
N-glycan trimming by glucosidase II is essential for Arabidopsis development (2009), Glycoconj. J., 26, 597-607.

Search for more literature for 3.2.1.207

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Schizosaccharomyces pombe mutants either glucosidase II-alpha or both glucosidase II-alpha and -beta minus, vector pTOTO, vector pREP3X, vector pSGP72	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
rsw3	Substitution of Ser599 by a Phe residue, inactivation of the alpha-subunit under non-permissive temperature because mutant is strongly temperature-sensitive	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	in most species the endoplasmic reticulum localization is mediated by the glucosidase II-beta subunit	Arabidopsis thaliana	5783	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
104000	-	deduced from cDNA sequences of Arabidopsis thaliana glucosidase II-alpha encoding gene	Arabidopsis thaliana
117000	-	molecular mass of protein extract of both glucosidase II-alpha and -beta minus Schizosaccharomyces pombe mutant cells after cells transformed with the cDNA sequences of Arabidopsis thaliana glucosidase II-alpha encoding gen and after deglycosylation	Arabidopsis thaliana
119000	-	western blot analysis of protein extract of both glucosidase II-alpha and -beta minus Schizosaccharomyces pombe mutant cells after cells transformed with the cDNA sequences of Arabidopsis thaliana glucosidase II-alpha encoding gene	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9FN05	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Glc2Man9GlcNAc2 + 2 H2O	-	Arabidopsis thaliana	Man9GlcNAc2 + 2 D-glucose	enzyme expression in Schizosaccharomyces pombe mutants either glucosidase II-alpha or both glucosidase II-alpha and -beta minus after cells transformed with the cDNA sequences of Arabidopsis thaliana glucosidase II-alpha encoding gene	?
GlcMan9GlcNAc + H2O	-	Arabidopsis thaliana	Man9GlcNAc + D-glucose	enzyme expression in Schizosaccharomyces pombe mutants either glucosidase II-alpha or both glucosidase II-alpha and -beta minus after cells transformed with the cDNA sequences of Arabidopsis thaliana glucosidase II-alpha encoding gene	?

Subunits

Subunits	Comment	Organism
heterodimer	alphabeta	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
glucosidase II	-	Arabidopsis thaliana
plant glucosidase	-	Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	-	non-permissive stability	Arabidopsis thaliana

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)