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Literature summary for 3.2.1.207 extracted from
- Endoplasmic Reticulum Glucosidase II is inhibited by its end products (2008), Biochemistry, 47, 10970-10980.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| calreticulin | 75 micromol, conversion of Glc2Man7-residue to Glc1Man7-residue | Rattus norvegicus | |
| calreticulin | in higher concentrations faster hydrolysis of Glc2Man9-residue | Rattus norvegicus | |
| GlcMan9GlcNAc2 | faster reaction | Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| calreticulin | slower hydrolysis of Glc1Man9-residue to Glc0Man9-residue | Rattus norvegicus | |
| Man7GlcNAc2 | - |
Rattus norvegicus | |
| Man9GlcNAc2 | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
N-[[2-carboxy-5-(4-methoxyphenyl)thiophen-3-yl]carbamoyl]-L-tyrosine | Glc2Man7GlcNAc2 | Rattus norvegicus | |
| 15 | 23 | N-[[2-carboxy-5-(4-methoxyphenyl)thiophen-3-yl]carbamoyl]-L-tyrosine | Glc2Man9GlcNAc2 | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.08 | - |
measured with substrate p-nitrophenyl-alpha-D-pyranosile | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glc2Man7GlcNAc2 + H2O | - |
Rattus norvegicus | GlcMan7GlcNAc2 + D-glucose | - |
? | |
| Glc2Man9GlcNAc2 + H2O | - |
Rattus norvegicus | GlcMan9GlcNAc2 + D-glucose | - |
? | |
| GlcMan7GlcNAc2 + H2O | - |
Rattus norvegicus | Man7GlcNAc2 + D-glucose | - |
? | |
| GlcMan9GlcNAc2 + H2O | - |
Rattus norvegicus | Man9GlcNAc2 + D-glucose | - |
? | |
| additional information | kinetic model for the interaction of glucosidase with calnexin/calreticulin | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoplasmic reticulum glucosidase II | - |
Rattus norvegicus |
| glucosidase II | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
incubation time is 10 min | Rattus norvegicus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 4 | - |
- |
Rattus norvegicus | Man7GlcNAc2 | |
| 40 | - |
- |
Rattus norvegicus | Man9GlcNAc2 | |
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Release 2023.1 (January 2023)
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