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Literature summary for 3.2.1.207 extracted from

  • Pelletier, M.F.; Marcil, A.; Sevigny, G.; Jakob, C.A.; Tessier, D.C.; Chevet, E.; Menard, R.; Bergeron, J.J.M.; Thomas, D.Y.
    The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo (2000), Glycobiology, 10, 815-827.
    View publication on PubMed
Search for more literature for 3.2.1.207

Cloned(Commentary)

Cloned (Comment) Organism
expressed with a baculovirus expression system in Sf9 cells, expressed in Cos7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D542N D542 required for catalytic activity Homo sapiens
D564N D564 required for catalytic activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
castanospermine
-
 Homo sapiens
deoxynojirimycin competitive, strong inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 p-nitrophenyl-alpha-D-glucopyranoside pH 7.5, 37°C, alpha1-beta complex Homo sapiens
0.52
-
 p-nitrophenyl-alpha-D-glucopyranoside pH 7.5, 37°C, alpha2-beta complex Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 SDS-PAGE, beta subunit Homo sapiens
110000
-
 SDS-PAGE, alpha1 subunit Homo sapiens
250000
-
 native PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Glc2Man9GlcNAc2 + H2O Homo sapiens involved in the Calnexin Cycle Man9GlcNAc2 + alpha-D-glucose
-
 ?
additional information Homo sapiens involved in early glycoprotein biogenesis, catalyzes the hydrolysis of two alpha-1,3-linked glucose residues present on all Asn-linked precursor oligosaccharides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P14314
-
-
Homo sapiens P14314 beta subunit
-
Homo sapiens Q14697
-
-
Homo sapiens Q14697 alpha1 isofrom of the alpha subunit, isoforms are formed by alternative splicing
-
Homo sapiens Q14697 alpha2 isoform of the alpha subunit, contains a 66 bp insert, isoforms are formed by alternative splicing
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant protein using His-tag Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + D-glucopyranose
-
 ?
Glc2Man9GlcNAc2 + H2O involved in the Calnexin Cycle Homo sapiens Man9GlcNAc2 + alpha-D-glucose
-
 ?
additional information involved in early glycoprotein biogenesis, catalyzes the hydrolysis of two alpha-1,3-linked glucose residues present on all Asn-linked precursor oligosaccharides Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer alpha, beta, two different isoforms of the alpha subunit identified, alpha1 is the short and alpha2 the long isoform, both isoforms form dimers with the beta subunit, only dimeric form shows catalytic activity Homo sapiens
dimer alpha, beta, two different isoforms of the alpha subunit identified, alpha1 is the short and alpha2 the long isoforms, both isoforms forms dimers with the beta subunit, only dimeric form shows catalytic activity Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
 deoxynojirimycin pH 7.5, 37°C Homo sapiens
0.015
-
 castanospermine pH 7.5, 37°C Homo sapiens
0.017
-
 castanospermine pH 7.5, 37°C Homo sapiens