Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | 0.2-1 mM, 110% of initial activity | Thermococcus kodakarensis | |
NaCl | 0.2-1 mM, 110% of initial activity | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q9UWR7 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62.7 | - |
substrate chitin, N-terminal catalytic domain, pH 5.0, 80°C | Thermococcus kodakarensis |
477 | - |
substrate ethylene glycol chitin, N-terminal catalytic domain, pH 5.0, 80°C | Thermococcus kodakarensis |
2490 | - |
substrate colloidal chitin, N-terminal catalytic domain, pH 5.0, 80°C | Thermococcus kodakarensis |
3160 | - |
substrate chitosan, 70% deacetylated, N-terminal catalytic domain, pH 5.0, 80°C | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | - |
Thermococcus kodakarensis | N,N'-diacetylchitobiose + ? | sole product of the N-terminal catalytic domain, the domain mainly hydrolyzes the second glycosidic bond from the nonreducing end of the oligomers | ? | |
colloidal chitin + H2O | - |
Thermococcus kodakarensis | N,N'-diacetylchitobiose + ? | - |
? | |
ethylene glycol chitin + H2O | - |
Thermococcus kodakarensis | N,N'-diacetylchitobiose | - |
? | |
partially N-acetylated chitosan + H2O | - |
Thermococcus kodakarensis | N,N'-diacetylchitobiose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChiA | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
stable for 60 min | Thermococcus kodakarensis |
90 | - |
half-life 5 min | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Thermococcus kodakarensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Thermococcus kodakarensis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme has a multidomain structure containing dual catalytic domains and triple chitin-binding domains.The N-terminal catalytic domain mainly hydrolyzes the second glycosidic bond from the nonreducing end of the oligomers, whereas the C-terminal domain randomly hydrolyzes glycosidic bonds other than the first bond from the nonreducing end. Both catalytic domains form diacetylchitobiose as a major end product and possess transglycosylation activity. The N-terminal catalytic domain exclusively liberates diacetylchitobiose, whereas reactions with the C-terminal domain lead to N-acetyl-chitooligosaccharides of various lengths. A synergistic effect is observed when chitin is degraded in the presence of both catalytic domains | Thermococcus kodakarensis |