Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5% loss of activity at 20 mM | Trichosanthes dioica | |
EDTA | 15% loss of activity at 20 mM | Trichosanthes dioica | |
Urea | 20% loss of activity at 8 M | Trichosanthes dioica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | enzyme activity increases significantly in the presence of 20 mM Mg2+ | Trichosanthes dioica | |
Mn2+ | enzyme activity increases significantly in the presence of 20 mM Mn2+ | Trichosanthes dioica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichosanthes dioica | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Trichosanthes dioica |
Purification (Comment) | Organism |
---|---|
QA-cellulose column chromatography | Trichosanthes dioica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Trichosanthes dioica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
colloidal chitin + H2O | - |
Trichosanthes dioica | N-acetyl D-glucosamine + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, SDS-PAGE | Trichosanthes dioica |
Synonyms | Comment | Organism |
---|---|---|
TdsC | - |
Trichosanthes dioica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | - |
Trichosanthes dioica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | more than 90% activity between 30 and 80°C | Trichosanthes dioica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
the enzyme retains 90% activity after 30 min at 90°C | Trichosanthes dioica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Trichosanthes dioica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | about 50% activity at pH 4.0, 100% activity at pH 5.0-8.0, about 90% activity at pH 9.0-10.0 | Trichosanthes dioica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | the enzyme remains stable after 30 min at pH 5.0-10.0, 80% activity is left after 30 min at pH 4.0 and 11.0 | Trichosanthes dioica |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme shows antifungal activity against Aspergillus niger and Trichoderma sp. | Trichosanthes dioica |