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Literature summary for 3.2.1.200 extracted from

  • Howard, M.B.; Ekborg, N.A.; Taylor, L.E.; Weiner, R.M.; Hutcheson, S.W.
    Chitinase B of "Microbulbifer degradans" 2-40 contains two catalytic domains with different chitinolytic activities (2004), J. Bacteriol., 186, 1297-1303.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information not inhibitory or activating: Mg2+, Mn2+, Ca2+, K+, EDTA, EGTA, or 1.0 M NaCl Saccharophagus degradans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 10 mM, 41% loss of activity Saccharophagus degradans
Hg2+ 10 mM, complete loss of activity Saccharophagus degradans
additional information not inhibitory or activating: Mg2+, Mn2+, Ca2+, K+, EDTA, EGTA, or 1.0 M NaCl Saccharophagus degradans
Ni2+ 10 mM, 36% loss of activity Saccharophagus degradans

Organism

Organism UniProt Comment Textmining
Saccharophagus degradans Q7PC53
-
-
Saccharophagus degradans DSM 17024 Q7PC53
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5900
-
substrate 4'-methylumbelliferyl-N,N',N''-triacetylchitobiose, aminoterminal catalytic domain, pH 7.5, 30°C Saccharophagus degradans
80000
-
substrate 4'-methylumbelliferyl-N,N'-diacetylchitobiose, aminoterminal catalytic domain, pH 7.5, 30°C Saccharophagus degradans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
-
Saccharophagus degradans methylumbelliferone + N,N',N''-triacetylchitotriose
-
?
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
-
Saccharophagus degradans DSM 17024 methylumbelliferone + N,N',N''-triacetylchitotriose
-
?
4-methylumbelliferyl N,N'-diacetylchitobioside + H2O
-
Saccharophagus degradans methylumbelliferone + N,N'-diacetylchitobiose
-
?
4-methylumbelliferyl N,N'-diacetylchitobioside + H2O
-
Saccharophagus degradans DSM 17024 methylumbelliferone + N,N'-diacetylchitobiose
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
Saccharophagus degradans N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
Saccharophagus degradans DSM 17024 N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
Saccharophagus degradans 2 N,N'-diacetylchitobiose sole product ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
Saccharophagus degradans DSM 17024 2 N,N'-diacetylchitobiose sole product ?

Synonyms

Synonyms Comment Organism
chitinase B
-
Saccharophagus degradans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Saccharophagus degradans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30
-
80% of maximum activity Saccharophagus degradans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 8
-
Saccharophagus degradans

General Information

General Information Comment Organism
physiological function chitinase B is predicted to contain two glycoside hydrolase family 18 (GH18) catalytic domains, both of which are catalytically active against chitin. The aminoterminal catalytic domain (GH18N) releases methylumbelliferone from 4'-methylumbelliferyl-N,N'-diacetylchitobiose and releases chitobiose from the nonreducing end of chitooligosaccharides. The C-terminal domain shows endochitinolytic activity. The domains function cooperatively to degrade chitin Saccharophagus degradans