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Literature summary for 3.2.1.20 extracted from
- Improved synthesis of isomaltooligosaccharides using immobilized alpha-glucosidase in organic-aqueous media (2017), Food Sci. Biotechnol., 26, 731-738 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | enzyme immobilization onto an epoxy-activated resin (Eupergit C), improved synthesis of isomaltooligosaccharides (IMOs) using immobilized alpha-glucosidase in organic-aqueous media, method optimization, overview. An immobilization efficiency of 79.61% is obtained under the condition of pH 6.0, ionic strength of 2.0 M, and 30 mg of protein/g of resin. The butyl acetate-aqueous biphasic system significantly improves the catalytic activity of the immobilized enzyme and the yield of IMOs. The highest yield of IMOs (50.83% w/w) is obtained at pH 4.5 and 55°C in a butyl acetate/buffer system (25:75 v/v) | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of transglucosylation activity of immobilized alpha-glucosidase | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | A0PCH8 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Aspergillus niger | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | immobilized recombinant alpha-glucosidase catalyzes maltose into isomaltooligosaccharides (IMOs) by its transglucosylation activity. The synthesis of IMOs is conducted using maltose as the substrate | Aspergillus niger | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AGL | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus niger |
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Release 2023.1 (January 2023)
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