Cloned (Comment) | Organism |
---|---|
gene Agl, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Pichia pastoris | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
N694A | site-directed mutagenesis, the mutant shows altered transglucosylation activity compared to the wild-type | Aspergillus niger |
N694F | site-directed mutagenesis, the mutant shows altered transglucosylation activity compared to the wild-type, the pH optimum is increased compared to the wild-type enzyme | Aspergillus niger |
N694L | site-directed mutagenesis, the mutant shows altered transglucosylation activity compared to the wild-type, the pH optimum is increased and the thermal stability slightly reduced compared to the wild-type enzyme | Aspergillus niger |
N694W | site-directed mutagenesis, the mutant shows altered transglucosylation activity compared to the wild-type | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics for transglucosylation of wild-type and mutant enzymes, overview | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | A0PCH8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Pichia pastoris by nickel affinity chromatography | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
120 | - |
enzyme mutant N694L, transglucosylation activity, pH 4.0, 37°C | Aspergillus niger |
152 | - |
enzyme mutant N694A, transglucosylation activity, pH 4.0, 37°C | Aspergillus niger |
155 | - |
enzyme mutant N694W, transglucosylation activity, pH 4.0, 37°C | Aspergillus niger |
185 | - |
enzyme mutant N694F, transglucosylation activity, pH 4.0, 37°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | - |
Aspergillus niger | 2 D-glucose | - |
? | |
additional information | transglucosylation activity by wild-type and Asn694 mutant enzymes forming isomaltooligosaccharides, overview. Isomaltose and panose are the predominant final products of wild-type alpha-glucosidase | Aspergillus niger | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | alpha-glucosidases structure comparisons, overview | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
AGL | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
wild-type enzyme | Aspergillus niger |
4.7 | - |
mutant N694F | Aspergillus niger |
4.8 | - |
mutant N694L | Aspergillus niger |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycoside hydrolase family 31, GH31 | Aspergillus niger |