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Literature summary for 3.2.1.20 extracted from

  • Casa-Villegas, M.; Marin-Navarro, J.; Polaina, J.
    Synthesis of isomaltooligosaccharides by Saccharomyces cerevisiae cells expressing Aspergillus niger alpha-glucosidase (2017), ACS omega, 2, 8062-8068 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.20

Cloned(Commentary)

Cloned (Comment) Organism
gene aglA, recombinant expression in Saccharomyces cerevisiae strain BY474, which lacks maltose, under control of a galactose-inducible promoter. Recombinant yeast cells expressing the aglA gene produce extracellular alpha-glucosidase activity about half of which appeared cell bound whereas the other half is released into the culture medium Aspergillus niger

Protein Variants

Protein Variants Comment Organism
additional information construction of a gene constructs in which the aglA gene is fused to glycosylphosphatidylinositol anchor sequences, from the yeast SED1 gene, that determine the covalent binding of the hybrid protein to the cell membrane, for that yeast cells can be used directly as the catalytic agent to produce isomaltooligosaccharides (IMOs). The resulting hybrid enzymes are stably attached to the cell surface. The cells from cultures of recombinant yeast strains expressing aglA-SED1 constructions can be used to produce IMOs in successive batches Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
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 Aspergillus niger
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-

Organism

Organism UniProt Comment Textmining
Aspergillus niger P56526
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information synthesis of panose or isomaltose by transglycosylation as a function of the glucosyl acceptor, panose is the main transglycosylation product after 8 h of incubation, whereas isomaltose is predominant after 24 h. Isomaltose also becomes predominant at shorter times if a mixture of maltose and glucose is used instead of maltose Aspergillus niger ?
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 ?

Synonyms

Synonyms Comment Organism
AglA
-
 Aspergillus niger

General Information

General Information Comment Organism
additional information isomaltose and panose docked at the active site of Aspergillus niger alpha-glucosidase involving the catalytic residues Asp490 and Asp660, putative nucleophile and acid/base catalytic residues, modelling, overview Aspergillus niger