Literature summary for 3.2.1.20 extracted from
Hwang, S.; Seo, S.; Park, I.; Choi, K.; Kim, D.; Cha, J.
Improvement of transglycosylation efficiency using a glycosynthase mutant derived from Thermoplasma acidophilum alpha-glucosidase (2012), Korean J. Microbiol. Biotechnol., 40, 104-110 .
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
mutant enzyme D408G, expression in Escherichia coli BL21(DE3) |
Thermoplasma acidophilum |
Protein Variants
Protein Variants |
Comment |
Organism |
D408G |
transglycosylation efficiency is improved. The mutant enzyme catalyzes the formation of a specific product from 4-nitrophenyl-alpha-D-glucopyranoside with a yield of 42.5% without further hydrolysis, while the wild-type enzyme produces two 4-nitrophenyl-alpha-D-glucopyranoside products at low yields |
Thermoplasma acidophilum |
Organism
Organism |
UniProt |
Comment |
Textmining |
Thermoplasma acidophilum |
Q9HLD0 |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
mutant enzyme D408G |
Thermoplasma acidophilum |
Synonyms
Synonyms |
Comment |
Organism |
AglA |
- |
Thermoplasma acidophilum |
Ta0298 |
- |
Thermoplasma acidophilum |