Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Thermoplasma acidophilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acarbose | competitive, pH 5.5, 80°C | Thermoplasma acidophilum | |
Aesculin | mixed type of noncompetitive inhibition, pH 5.5, 80°C | Thermoplasma acidophilum | |
CuCl2 | 1 mM, 65% loss of activity | Thermoplasma acidophilum | |
EDTA | 1 mM, 7% loss of activity | Thermoplasma acidophilum | |
HgCl2 | 1 mM, 96% loss of activity | Thermoplasma acidophilum | |
N-bromosuccinimide | 1 mM, 92% loss of activity | Thermoplasma acidophilum | |
NiCl2 | 1 mM, 16% loss of activity | Thermoplasma acidophilum | |
p-chloromercuribenzoate | 1 mM, 5% loss of activity | Thermoplasma acidophilum | |
SDS | 1 mM, complete inactivation | Thermoplasma acidophilum | |
ZnCl2 | 1 mM, 72% loss of activity | Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
maltotriose | pH 5.5, 80°C | Thermoplasma acidophilum | |
0.65 | - |
maltose | pH 5.5, 80°C | Thermoplasma acidophilum | |
0.67 | - |
maltotetraose | pH 5.5, 80°C | Thermoplasma acidophilum | |
1.24 | - |
kojibiose | pH 5.5, 80°C | Thermoplasma acidophilum | |
1.77 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 80°C | Thermoplasma acidophilum | |
4.25 | - |
isomaltose | pH 5.5, 80°C | Thermoplasma acidophilum | |
6.6 | - |
panose | pH 5.5, 80°C | Thermoplasma acidophilum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Thermoplasma acidophilum | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MnCl2 | 1 mM, 1.6fold activation | Thermoplasma acidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
409000 | - |
gel filtration | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | Q9HLD0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Thermoplasma acidophilum | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
isomaltose + H2O | hydrolyzed to e lesser extent than maltose | Thermoplasma acidophilum | 2 D-glucose | - |
? | |
kojibiose + H2O | hydrolyzed to e lesser extent than maltose | Thermoplasma acidophilum | 2 D-glucose | - |
? | |
maltooligosaccharide + H2O | the enzyme shows highest specificity for maltose and decreasing values of catalytic efficiency (kcat/Km) toward higher maltooligosaccahrides. AglA specifically liberates glucose from the non-reducing end | Thermoplasma acidophilum | ? | - |
? | |
maltopentaose + H2O | - |
Thermoplasma acidophilum | D-glucose + ? | - |
? | |
maltose + H2O | - |
Thermoplasma acidophilum | 2 D-glucose | - |
? | |
maltotetraose + H2O | - |
Thermoplasma acidophilum | D-glucose + ? | - |
? | |
maltotriose + H2O | - |
Thermoplasma acidophilum | D-glucose + ? | - |
? | |
additional information | structural analyses of two arbutin transglucosylation products of the transglucosylation reaction with arbutin and maltose indicates that the glucose unit of maltose was transferred to the C-3 and C-6 position in the glucose moiety of arbutin | Thermoplasma acidophilum | ? | - |
? | |
nigerose + H2O | hydrolyzed to e lesser extent than maltose | Thermoplasma acidophilum | 2 D-glucose | - |
? | |
panose + H2O | - |
Thermoplasma acidophilum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
pentamer | 5 * 82000, SDS-PAGE | Thermoplasma acidophilum |
Synonyms | Comment | Organism |
---|---|---|
AglA | - |
Thermoplasma acidophilum |
Ta0298 | - |
Thermoplasma acidophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermoplasma acidophilum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | 60°C: about 55% of maximal activity, 90°C: about 75% of maximal activity | Thermoplasma acidophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
16.8 h, enzyme retains its full activity | Thermoplasma acidophilum |
85 | - |
half-life: 1.4 h | Thermoplasma acidophilum |
90 | - |
half-life: 12 min | Thermoplasma acidophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22.9 | - |
isomaltose | pH 5.5, 80°C | Thermoplasma acidophilum | |
35.9 | - |
panose | pH 5.5, 80°C | Thermoplasma acidophilum | |
59.7 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 80°C | Thermoplasma acidophilum | |
85.5 | - |
maltotetraose | pH 5.5, 80°C | Thermoplasma acidophilum | |
90.2 | - |
maltotriose | pH 5.5, 80°C | Thermoplasma acidophilum | |
127 | - |
kojibiose | pH 5.5, 80°C | Thermoplasma acidophilum | |
143 | - |
maltose | pH 5.5, 80°C | Thermoplasma acidophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
substrate: 4-nitrophenyl alpha-D-glucopyranoside | Thermoplasma acidophilum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | pH 4.0: about 55% of maximal activity, pH 7.0: about 70% of maximal activity | Thermoplasma acidophilum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00299 | - |
acarbose | pH 5.5, 80°C | Thermoplasma acidophilum | |
0.0043 | - |
Aesculin | pH 5.5, 80°C | Thermoplasma acidophilum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermoplasma acidophilum | calculated from sequence | - |
5.29 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.4 | - |
isomaltose | pH 5.5, 80°C | Thermoplasma acidophilum | |
5.4 | - |
panose | pH 5.5, 80°C | Thermoplasma acidophilum | |
33.7 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 80°C | Thermoplasma acidophilum | |
103 | - |
kojibiose | pH 5.5, 80°C | Thermoplasma acidophilum | |
128 | - |
maltotetraose | pH 5.5, 80°C | Thermoplasma acidophilum | |
194 | - |
maltotriose | pH 5.5, 80°C | Thermoplasma acidophilum | |
220 | - |
maltose | pH 5.5, 80°C | Thermoplasma acidophilum |