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Literature summary for 3.2.1.20 extracted from

  • Seo, S.; Choi, K.; Hwang, S.; Kim, J.; Park, C.; Rho, J.; Cha, J.
    Characterization of the catalytic and kinetic properties of a thermostable Thermoplasma acidophilum alpha-glucosidase and its transglucosylation reaction with arbutin (2011), J. Mol. Catal. B, 72, 305-312 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Thermoplasma acidophilum

Inhibitors

Inhibitors Comment Organism Structure
acarbose competitive, pH 5.5, 80°C Thermoplasma acidophilum
Aesculin mixed type of noncompetitive inhibition, pH 5.5, 80°C Thermoplasma acidophilum
CuCl2 1 mM, 65% loss of activity Thermoplasma acidophilum
EDTA 1 mM, 7% loss of activity Thermoplasma acidophilum
HgCl2 1 mM, 96% loss of activity Thermoplasma acidophilum
N-bromosuccinimide 1 mM, 92% loss of activity Thermoplasma acidophilum
NiCl2 1 mM, 16% loss of activity Thermoplasma acidophilum
p-chloromercuribenzoate 1 mM, 5% loss of activity Thermoplasma acidophilum
SDS 1 mM, complete inactivation Thermoplasma acidophilum
ZnCl2 1 mM, 72% loss of activity Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
maltotriose pH 5.5, 80°C Thermoplasma acidophilum
0.65
-
maltose pH 5.5, 80°C Thermoplasma acidophilum
0.67
-
maltotetraose pH 5.5, 80°C Thermoplasma acidophilum
1.24
-
kojibiose pH 5.5, 80°C Thermoplasma acidophilum
1.77
-
4-nitrophenyl alpha-D-glucopyranoside pH 5.5, 80°C Thermoplasma acidophilum
4.25
-
isomaltose pH 5.5, 80°C Thermoplasma acidophilum
6.6
-
panose pH 5.5, 80°C Thermoplasma acidophilum

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Thermoplasma acidophilum 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
MnCl2 1 mM, 1.6fold activation Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
409000
-
gel filtration Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum Q9HLD0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
Thermoplasma acidophilum 4-nitrophenol + alpha-D-glucopyranose
-
?
isomaltose + H2O hydrolyzed to e lesser extent than maltose Thermoplasma acidophilum 2 D-glucose
-
?
kojibiose + H2O hydrolyzed to e lesser extent than maltose Thermoplasma acidophilum 2 D-glucose
-
?
maltooligosaccharide + H2O the enzyme shows highest specificity for maltose and decreasing values of catalytic efficiency (kcat/Km) toward higher maltooligosaccahrides. AglA specifically liberates glucose from the non-reducing end Thermoplasma acidophilum ?
-
?
maltopentaose + H2O
-
Thermoplasma acidophilum D-glucose + ?
-
?
maltose + H2O
-
Thermoplasma acidophilum 2 D-glucose
-
?
maltotetraose + H2O
-
Thermoplasma acidophilum D-glucose + ?
-
?
maltotriose + H2O
-
Thermoplasma acidophilum D-glucose + ?
-
?
additional information structural analyses of two arbutin transglucosylation products of the transglucosylation reaction with arbutin and maltose indicates that the glucose unit of maltose was transferred to the C-3 and C-6 position in the glucose moiety of arbutin Thermoplasma acidophilum ?
-
?
nigerose + H2O hydrolyzed to e lesser extent than maltose Thermoplasma acidophilum 2 D-glucose
-
?
panose + H2O
-
Thermoplasma acidophilum ?
-
?

Subunits

Subunits Comment Organism
pentamer 5 * 82000, SDS-PAGE Thermoplasma acidophilum

Synonyms

Synonyms Comment Organism
AglA
-
Thermoplasma acidophilum
Ta0298
-
Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Thermoplasma acidophilum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 90 60°C: about 55% of maximal activity, 90°C: about 75% of maximal activity Thermoplasma acidophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
16.8 h, enzyme retains its full activity Thermoplasma acidophilum
85
-
half-life: 1.4 h Thermoplasma acidophilum
90
-
half-life: 12 min Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22.9
-
isomaltose pH 5.5, 80°C Thermoplasma acidophilum
35.9
-
panose pH 5.5, 80°C Thermoplasma acidophilum
59.7
-
4-nitrophenyl alpha-D-glucopyranoside pH 5.5, 80°C Thermoplasma acidophilum
85.5
-
maltotetraose pH 5.5, 80°C Thermoplasma acidophilum
90.2
-
maltotriose pH 5.5, 80°C Thermoplasma acidophilum
127
-
kojibiose pH 5.5, 80°C Thermoplasma acidophilum
143
-
maltose pH 5.5, 80°C Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
substrate: 4-nitrophenyl alpha-D-glucopyranoside Thermoplasma acidophilum

pH Range

pH Minimum pH Maximum Comment Organism
4 7 pH 4.0: about 55% of maximal activity, pH 7.0: about 70% of maximal activity Thermoplasma acidophilum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00299
-
acarbose pH 5.5, 80°C Thermoplasma acidophilum
0.0043
-
Aesculin pH 5.5, 80°C Thermoplasma acidophilum

pI Value

Organism Comment pI Value Maximum pI Value
Thermoplasma acidophilum calculated from sequence
-
5.29

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.4
-
isomaltose pH 5.5, 80°C Thermoplasma acidophilum
5.4
-
panose pH 5.5, 80°C Thermoplasma acidophilum
33.7
-
4-nitrophenyl alpha-D-glucopyranoside pH 5.5, 80°C Thermoplasma acidophilum
103
-
kojibiose pH 5.5, 80°C Thermoplasma acidophilum
128
-
maltotetraose pH 5.5, 80°C Thermoplasma acidophilum
194
-
maltotriose pH 5.5, 80°C Thermoplasma acidophilum
220
-
maltose pH 5.5, 80°C Thermoplasma acidophilum