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Literature summary for 3.2.1.20 extracted from
- Enzymatic properties of a thermostable alpha-glucosidase from acidothermophilic crenarchaeon Sulfolobus tokodaii strain 7 (2013), J. Microbiol. Biotechnol., 23, 56-63.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme can be potentially useful for starch hydrolysis as well for the novel synthesis of oligosaccharides in industry | Sulfurisphaera tokodaii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as a fusion protein in Escherichia coli | Sulfurisphaera tokodaii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.33 | - |
maltopentaose | pH 4.0, 80°C | Sulfurisphaera tokodaii |  |
| 2.78 | - |
maltotetraose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 3.11 | - |
maltotriose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 3.18 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 4.4 | - |
maltohexaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 5.09 | - |
maltoheptaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 5.78 | - |
panose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 10.9 | - |
maltose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 17.3 | - |
isomaltose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70500 | - |
x * 70500, SDS-PAGE | Sulfurisphaera tokodaii |
| 75600 | - |
x * 75600, calculated from sequence | Sulfurisphaera tokodaii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfurisphaera tokodaii | F9VPH0 | - |
- |
| Sulfurisphaera tokodaii 7 | F9VPH0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfurisphaera tokodaii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.7 | - |
pH 4.0, 80°C | Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Sulfurisphaera tokodaii | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
| isomaltose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
| maltoheptaose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
| maltoheptaose + H2O | - |
Sulfurisphaera tokodaii 7 | ? | - |
? | |
| maltohexaose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
| maltohexaose + H2O | - |
Sulfurisphaera tokodaii 7 | ? | - |
? | |
| maltopentaose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
| maltopentaose + H2O | - |
Sulfurisphaera tokodaii 7 | ? | - |
? | |
| maltose + H2O | - |
Sulfurisphaera tokodaii | 2 D-glucose | - |
? | |
| maltotetraose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
| maltotetraose + H2O | - |
Sulfurisphaera tokodaii 7 | ? | - |
? | |
| maltotriose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
| additional information | exclusively hydrolyzes alpha-1,4-glycosidic linkages of oligosaccharides in an exo-type manner, with highest catalytic efficiency toward maltotriose. The enzyme also displays transglycosylation activity, converting maltose to isomaltose, panose, maltotriose, isomaltotriose, etc. | Sulfurisphaera tokodaii | ? | - |
? | |
| additional information | exclusively hydrolyzes alpha-1,4-glycosidic linkages of oligosaccharides in an exo-type manner, with highest catalytic efficiency toward maltotriose. The enzyme also displays transglycosylation activity, converting maltose to isomaltose, panose, maltotriose, isomaltotriose, etc. | Sulfurisphaera tokodaii 7 | ? | - |
? | |
| panose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70500, SDS-PAGE | Sulfurisphaera tokodaii |
| ? | x * 75600, calculated from sequence | Sulfurisphaera tokodaii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| st2525 | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
assay at | Sulfurisphaera tokodaii |
| 95 | - |
- |
Sulfurisphaera tokodaii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 110 | 75°C: about 50% of maximal activity, 110°C: about 75% of maximal activity | Sulfurisphaera tokodaii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
half-life: 144 min | Sulfurisphaera tokodaii |
| 100 | - |
half-life: 40.1 min | Sulfurisphaera tokodaii |
| 105 | - |
half-life: 7.75 min | Sulfurisphaera tokodaii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0175 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.02 | - |
maltopentaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.02 | - |
maltohexaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.02 | - |
maltoheptaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.02 | - |
isomaltose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.02 | - |
panose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.03 | - |
maltotetraose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.04 | - |
maltotriose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.11 | - |
maltose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Sulfurisphaera tokodaii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 5 | pH 3.5: about 95% of maximal activity, pH 5.0: about 70% of maximal activity, potassium phosphate buffer | Sulfurisphaera tokodaii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | 4°C, 30 min, the enzyme retains at least 85% of its maximal activity | Sulfurisphaera tokodaii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0013 | - |
isomaltose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.0034 | - |
panose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.0038 | - |
maltoheptaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.0052 | - |
maltohexaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.0055 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.0097 | - |
maltose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.0098 | - |
maltopentaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.0113 | - |
maltotetraose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
| 0.0136 | - |
maltotriose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
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