Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.446 | - |
p-nitrophenyl-alpha-D-glucopyranoside | - |
Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106000 | - |
SDS-PAGE, after deglycosylation | Aspergillus niger |
145000 | - |
SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | A2QJF7 | - |
- |
Purification (Comment) | Organism |
---|---|
ultrafiltration, ethanol fraction and hydrophobic interaction chromatography | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.52 | - |
- |
Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | assay at pH 5.5, 50°C, reaction terminated by sodium carbonate solution | Aspergillus niger | 4-nitrophenol + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucosidase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | - |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
50% remaining activity after 72 h at 50°C, 50% remaining activity after 3 h at 60°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | - |
Aspergillus niger |