Literature summary for 3.2.1.20 extracted from

Frade-Perez, M.D.; Hernandez-Cervantes, A.; Flores-Carreon, A.; Mora-Montes, H.M.
Biochemical characterization of Candida albicans alpha-glucosidase I heterologously expressed in Escherichia coli (2010), Antonie van Leeuwenhoek, 98, 291-298.

Search for more literature for 3.2.1.20

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Candida albicans

Inhibitors

Inhibitors	Comment	Organism	Structure
1-deoxynojirimycin	-	Candida albicans
australine	0.02 mM, 39% inhibition of enzyme activity	Candida albicans
bromoconduritol	0.02 mM, 22% inhibition of enzyme acitvity	Candida albicans
castanospermine	-	Candida albicans
D-glucose	-	Candida albicans
D-mannose	-	Candida albicans
N-ethylmaleimide	-	Candida albicans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.133	-	4-Methylumbelliferyl-alpha-D-glucopyranoside	-	Candida albicans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	-	Candida albicans	5783	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	SDS-PAGE, recombinant enzyme	Candida albicans

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	Q2V621	-	-

Purification (Commentary)

Purification (Comment)	Organism
anion exchange chromatography in DEAE Bio-Gel, Sephadex G-25 column	Candida albicans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl-alpha-D-glucopyranoside + H2O	assay at pH 7.0, 37°C, 30 min incubation	Candida albicans	4-methylumbelliferone + alpha-D-glucose	-	?

Synonyms

Synonyms	Comment	Organism
alpha-glucosidase I	-	Candida albicans
CWH41	-	Candida albicans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Candida albicans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Candida albicans

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0027	-	-	Candida albicans	castanospermine
0.0029	-	-	Candida albicans	1-deoxynojirimycin
2.2	-	-	Candida albicans	N-ethylmaleimide
2.9	-	-	Candida albicans	D-glucose
9.1	-	-	Candida albicans	D-mannose

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Release 2023.1 (January 2023)