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Literature summary for 3.2.1.20 extracted from

  • Shirai, T.; Hung, V.S.; Morinaka, K.; Kobayashi, T.; Ito, S.
    Crystal structure of GH13 alpha-glucosidase GSJ from one of the deepest sea bacteria (2008), Proteins, 73, 126-133.
    View publication on PubMed
Search for more literature for 3.2.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Bacillus subtilis ISW1214 cells Geobacillus sp. HTA-462

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the enzyme is determined to a 2.0 A resolution, by X-ray crystallography Geobacillus sp. HTA-462

Inhibitors

Inhibitors Comment Organism Structure
D-glucono-1,5-lactone
-
 Geobacillus sp. HTA-462

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Geobacillus sp. HTA-462

Organism

Organism UniProt Comment Textmining
Geobacillus sp. HTA-462 Q33E90
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Geobacillus sp. HTA-462

Purification (Commentary)

Purification (Comment) Organism
GSJ is purified to homogeneity, gel filtration Geobacillus sp. HTA-462

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oligomaltose + H2O broad ability in transglycosylation activity on nonsugar acceptors Geobacillus sp. HTA-462 D-glucose
-
 ?

Subunits

Subunits Comment Organism
homodimer GSJ forms a homodimer under low-ionic strength conditions, while all the structure-determined homologues are supposed to be monomeric proteins Geobacillus sp. HTA-462
More comparisons of the GSJ structure with that of other GH13 enzymes with different catalytic activities reveals that the catalytic cleft of GSJ is widely opened when compared with the homologues Geobacillus sp. HTA-462

Synonyms

Synonyms Comment Organism
alpha-glucosidase
-
 Geobacillus sp. HTA-462
GH13 alpha-glucosidase
-
 Geobacillus sp. HTA-462
GSJ
-
 Geobacillus sp. HTA-462