Cloned (Comment) | Organism |
---|---|
genes gluA and gluB, BAC library construction, DNA and amino acid sequence determination and analysis, expression analysis, expression of the isozymes in Escherichia coli strain XL 1-Blue cytosol | Cronobacter sakazakii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cronobacter sakazakii | - |
two isozymes encoded by genes gluA and gluB | - |
Cronobacter sakazakii 858 | - |
two isozymes encoded by genes gluA and gluB | - |
Purification (Comment) | Organism |
---|---|
recombinant isozymes from Eschericia coli strain XL 1-Blue cytosol | Cronobacter sakazakii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-D-glucopyranoside + H2O | - |
Cronobacter sakazakii | 4-methylumbelliferol + alpha-D-glucose | - |
? | |
4-methylumbelliferyl-alpha-D-glucopyranoside + H2O | - |
Cronobacter sakazakii 858 | 4-methylumbelliferol + alpha-D-glucose | - |
? |