Cloned (Comment) | Organism |
---|---|
gene agl97, expression of functional His-tagged full-length enzyme in Pichia pastoris, production by high cell-density fermentation | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
maltose | pH 4.5, 37°C, recombinant enzyme | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92000 | - |
wild-type enzyme, gel filtration | Hordeum vulgare |
100000 | - |
recombinant enzyme, gel filtration | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | Hordeum vulgare | - |
alpha-D-glucose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | Q9LLY2 | gene agl97 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 25fold from Pichia pastoris by dia- and ultrafiltration, nickel affinity chromatography and gel filtration | Hordeum vulgare |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.6 | - |
purified recombinant enzyme | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | - |
Hordeum vulgare | alpha-D-glucose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, recombinant enzyme, SDS-PAGE | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
high pI alpha-glucosidase | - |
Hordeum vulgare |
More | the enzyme belongs to the alpha-glucosidase family 31 | Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Hordeum vulgare |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
81.7 | - |
maltose | pH 4.5, 37°C, recombinant enzyme | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Hordeum vulgare |