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Literature summary for 3.2.1.20 extracted from
- Purification and characterization of the hyper-glycosylated extracellular alpha-glucosidase from Schizosaccharomyces pombe (2005), Enzyme Microb. Technol., 37, 472-480.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| proteolysis susceptibility is descended by enzymatical deglycosylation | Schizosaccharomyces pombe |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Schizosaccharomyces pombe | |
| 4.9 | - |
phenyl alpha-glucoside | pH 4.5, 35°C | Schizosaccharomyces pombe |  |
| 6.3 | - |
maltose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 6.7 | - |
maltotriose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 7.1 | - |
maltotetraose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 9.9 | - |
Phenyl alpha-maltoside | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 14 | - |
maltopentaose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 14 | - |
isomaltose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 19 | - |
kojibiose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 43 | - |
maltohexaose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 55 | - |
nigerose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 62 | - |
panose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 76 | - |
maltoheptaose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Schizosaccharomyces pombe | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 106139 | - |
1 * 110000, deglycosylated enzyme, SDS-PAGE, 1 * 106139, amino acid sequence calculation | Schizosaccharomyces pombe |
| 1120000 | - |
- |
Schizosaccharomyces pombe |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + H2O | Schizosaccharomyces pombe | - |
2 alpha-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | Q9C0Y4 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the extracellular enzyme is hyperglycosylated and contains 88% carbohydrate, mainly N-glycosylation, overview, deglycosylation decreases the heat stability and susceptibility to proteolysis | Schizosaccharomyces pombe |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from culture supernatant by ethanol precipitation, anion exchange chromatography, and gel filtration | Schizosaccharomyces pombe |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| maltotetraose + 3 H2O = 4 alpha-D-glucose | the enzyme prefers short substrates, e.g. maltose and maltotriose, to longer substrates, and hydrolyzes alpha-1,4-glucosidic linkages, but also acts on alpha-1,2-, alpha-1,3-, and alpha-1,6-glucosidic linkages, the catalytic site contains three subsites with different affinities | Schizosaccharomyces pombe |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Schizosaccharomyces pombe | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | 2.5 | purified enzyme | Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isomaltose + H2O | low activity | Schizosaccharomyces pombe | 2 alpha-D-glucose | - |
? | |
| kojibiose + H2O | low activity | Schizosaccharomyces pombe | alpha-D-glucose + D-glucose | - |
? | |
| maltoheptaose + 6 H2O | low activity | Schizosaccharomyces pombe | 7 alpha-D-glucose | - |
? | |
| maltohexaose + 5 H2O | low activity | Schizosaccharomyces pombe | 6 alpha-D-glucose | - |
? | |
| maltopentaose + 4 H2O | - |
Schizosaccharomyces pombe | 5 alpha-D-glucose | - |
? | |
| maltose + H2O | - |
Schizosaccharomyces pombe | 2 alpha-D-glucose | - |
? | |
| maltotetraose + 3 H2O | - |
Schizosaccharomyces pombe | 4 alpha-D-glucose | - |
? | |
| maltotriose + 2 H2O | - |
Schizosaccharomyces pombe | 3 alpha-D-glucose | - |
? | |
| additional information | the enzyme prefers short substrates, e.g. maltose and maltotriose, to longer substrates, and hydrolyzes alpha-1,4-glucosidic linkages, but also acts on alpha-1,2-, alpha-1,3-, and alpha-1,6-glucosidic linkages, substrate specificity, soluble starch is a poor substrate, the catalytic site contains three subsites, subsite affinity, overview | Schizosaccharomyces pombe | ? | - |
? | |
| nigerose + H2O | alpha-1,3-linkage, very low activity | Schizosaccharomyces pombe | alpha-D-glucose + D-glucose | - |
? | |
| panose + H2O | low activity | Schizosaccharomyces pombe | maltose + alpha-D-glucose | - |
? | |
| phenyl alpha-glucoside + H2O | low activity | Schizosaccharomyces pombe | alpha-D-glucose + phenol | - |
? | |
| phenyl alpha-maltoside + H2O | preferred substrate, stereospecific production of alpha-D-glucose which spontaneously mutarotates to beta-glucose | Schizosaccharomyces pombe | alpha-D-glucose + ? | - |
? | |
| soluble starch + H2O | - |
Schizosaccharomyces pombe | alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 110000, deglycosylated enzyme, SDS-PAGE, 1 * 106139, amino acid sequence calculation | Schizosaccharomyces pombe |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Schizosaccharomyces pombe |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
heat stability and is descended by enzymatical deglycosylation | Schizosaccharomyces pombe |
| 37 | - |
deglycosylated enzyme is labile above | Schizosaccharomyces pombe |
| 40 | - |
15 min, native enzyme is stable up to | Schizosaccharomyces pombe |
| 50 | - |
15 min, inactivation | Schizosaccharomyces pombe |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 111 | - |
maltotetraose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 139 | - |
Phenyl alpha-maltoside | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 216 | - |
isomaltose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 433 | - |
kojibiose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 435 | - |
nigerose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 704 | - |
panose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 709 | - |
maltose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 810 | - |
maltopentaose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 874 | - |
maltohexaose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 994 | - |
maltotriose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 1020 | - |
maltoheptaose | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 1171 | - |
Phenyl alpha-maltoside | pH 4.5, 35°C | Schizosaccharomyces pombe | |
| 1171 | - |
phenyl alpha-glucoside | pH 4.5, 35°C | Schizosaccharomyces pombe | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Schizosaccharomyces pombe |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH-profile | Schizosaccharomyces pombe |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | 4°C, 24 h, native and deglycosylated enzyme, stable | Schizosaccharomyces pombe |
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