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Literature summary for 3.2.1.20 extracted from
- Characterization of thermostable alpha-glucosidase from Clostridium thermosulfuricum 39E (1991), Appl. Microbiol. Biotechnol., 35, 568-571.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acarbose | - |
Thermoanaerobacter thermohydrosulfuricus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
maltotriose | - |
Thermoanaerobacter thermohydrosulfuricus | |
| 0.58 | - |
maltotriose | - |
Thermoanaerobacter thermohydrosulfuricus | |
| 1.72 | - |
panose | - |
Thermoanaerobacter thermohydrosulfuricus | |
| 1.85 | - |
maltose | - |
Thermoanaerobacter thermohydrosulfuricus | |
| 2.95 | - |
isomaltose | - |
Thermoanaerobacter thermohydrosulfuricus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacter thermohydrosulfuricus | - |
39E | - |
| Thermoanaerobacter thermohydrosulfuricus 39E | - |
39E | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Thermoanaerobacter thermohydrosulfuricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Thermoanaerobacter thermohydrosulfuricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isomaltose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | 2 alpha-D-glucose | - |
? | |
| isomaltose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus 39E | 2 alpha-D-glucose | - |
? | |
| isomaltotriose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | alpha-D-glucose | - |
? | |
| isomaltotriose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus 39E | alpha-D-glucose | - |
? | |
| maltohexaose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltopentaose + D-glucose | - |
? | |
| maltose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | alpha-D-glucose + D-glucose | - |
? | |
| maltose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus 39E | alpha-D-glucose + D-glucose | - |
? | |
| maltotetraose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | alpha-D-glucose | - |
? | |
| maltotetraose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus 39E | alpha-D-glucose | - |
? | |
| maltotriose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltose + D-glucose | - |
? | |
| maltotriose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus 39E | maltose + D-glucose | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | 4-nitrophenol + D-glucose | - |
? | |
| panose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltose + alpha-D-glucose | - |
? | |
| phenyl alpha-D-glucoside + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | phenol + alpha-D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Thermoanaerobacter thermohydrosulfuricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48 | 80 | 48°C: about 50% of maximal activity, 80°C: about 95% of maximal activity | Thermoanaerobacter thermohydrosulfuricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life: 46 h | Thermoanaerobacter thermohydrosulfuricus |
| 70 | - |
half-life: 110 min | Thermoanaerobacter thermohydrosulfuricus |
| 75 | - |
half-life: 35 min | Thermoanaerobacter thermohydrosulfuricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | - |
Thermoanaerobacter thermohydrosulfuricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6 | pH 4.5: 90% of maximal activity, pH 6.0: 94% of maximal activity | Thermoanaerobacter thermohydrosulfuricus |
| 5 | 5.5 | - |
Thermoanaerobacter thermohydrosulfuricus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | most stable at | Thermoanaerobacter thermohydrosulfuricus |
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