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BRENDA support

Literature summary for 3.2.1.2 extracted from

  • Rasouli, N.; Sohrabi, N.; Zamani, M.
    Influence of a novel magnetic recoverable support on kinetic, stability and activity of beta-amylase enzyme (2016), Phys. Chem. Res., 4, 271-283 .
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
additional information covalent immobilization of beta-amylase enzyme on the surface of modified magnetic nanoparticles (ZnFe2O4-SiO2-NH2). The magnetic nanoparticles of ZnFe2O4 are synthesized by chemical co-precipitation method, and then tetraethyl orthosilicate (TEOS) and 3-aminopropyltriethoxy silane (APTES) are used for modification of ZnFe2O4 nanoparticles with silica and amine groups (ZnFe2O4-SiO2-NH2, classical Stoeber method). The aminated surface of ZnFe2O4 nanoparticles is exposed to beta-amylase immobilization using trichlorotriazine (TCT) as covalent agent. The immobilized beta-amylase enzyme is characterized by techniques such as Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), powder X-ray diffraction (XRD) and energy dispersive X-ray analysis (EDAX). The immobilized enzyme shows increased catalytic activity and efficiency, as well as thermal stability compared to free enzyme. Highest activity at pH 7.0 and 40°C. Method evaluation and kinetics, overview Ipomoea batatas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, modelling Ipomoea batatas
5.64
-
starch immobilized enzyme, pH 7.0, 40°C Ipomoea batatas
7.49
-
starch free enzyme, pH 7.0, 40°C Ipomoea batatas

Organism

Organism UniProt Comment Textmining
Ipomoea batatas P10537
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Ipomoea batatas
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O soluble starch Ipomoea batatas ?
-
?

Synonyms

Synonyms Comment Organism
1,4-alpha-D-glucan maltohydrolase UniProt Ipomoea batatas
beta amylase
-
Ipomoea batatas
Bmy1
-
Ipomoea batatas

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
free and immobilized enzyme Ipomoea batatas

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 the immobilized enzyme shows over 70% of maximal activity within this range, while the free enzyme only shows over 50% of maximal activity within this range Ipomoea batatas

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
free and immobilized enzyme Ipomoea batatas

pH Range

pH Minimum pH Maximum Comment Organism
4 8 immobilized enzyme, over 50% of maximal activity within this range Ipomoea batatas
5 7.5 free enzyme, over 50% of maximal activity within this range Ipomoea batatas