Protein Variants | Comment | Organism |
---|---|---|
additional information | covalent immobilization of beta-amylase enzyme on the surface of modified magnetic nanoparticles (ZnFe2O4-SiO2-NH2). The magnetic nanoparticles of ZnFe2O4 are synthesized by chemical co-precipitation method, and then tetraethyl orthosilicate (TEOS) and 3-aminopropyltriethoxy silane (APTES) are used for modification of ZnFe2O4 nanoparticles with silica and amine groups (ZnFe2O4-SiO2-NH2, classical Stoeber method). The aminated surface of ZnFe2O4 nanoparticles is exposed to beta-amylase immobilization using trichlorotriazine (TCT) as covalent agent. The immobilized beta-amylase enzyme is characterized by techniques such as Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), powder X-ray diffraction (XRD) and energy dispersive X-ray analysis (EDAX). The immobilized enzyme shows increased catalytic activity and efficiency, as well as thermal stability compared to free enzyme. Highest activity at pH 7.0 and 40°C. Method evaluation and kinetics, overview | Ipomoea batatas |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, modelling | Ipomoea batatas | |
5.64 | - |
starch | immobilized enzyme, pH 7.0, 40°C | Ipomoea batatas | |
7.49 | - |
starch | free enzyme, pH 7.0, 40°C | Ipomoea batatas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ipomoea batatas | P10537 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Ipomoea batatas | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | soluble starch | Ipomoea batatas | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1,4-alpha-D-glucan maltohydrolase | UniProt | Ipomoea batatas |
beta amylase | - |
Ipomoea batatas |
Bmy1 | - |
Ipomoea batatas |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
free and immobilized enzyme | Ipomoea batatas |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the immobilized enzyme shows over 70% of maximal activity within this range, while the free enzyme only shows over 50% of maximal activity within this range | Ipomoea batatas |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
free and immobilized enzyme | Ipomoea batatas |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | immobilized enzyme, over 50% of maximal activity within this range | Ipomoea batatas |
5 | 7.5 | free enzyme, over 50% of maximal activity within this range | Ipomoea batatas |