Literature summary for 3.2.1.2 extracted from

Sagu, S.T.; Nso, E.J.; Homann, T.; Kapseu, C.; Rawel, H.M.
Extraction and purification of beta-amylase from stems of Abrus precatorius by three phase partitioning (2015), Food Chem., 183, 144-153 .

Application

Application	Comment	Organism
industry	amylases are important industrial enzymes used in starch liquefaction to produce glucose, fructose and maltose and also in brewing, baking, textile, paper, detergent and sugar industries	Abrus precatorius

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Km and Vmax values are 79.37mg/ml and 5.13 U/ml, respectively, at 50°C, pH 6.0, Michaelis-Menten kinetics	Abrus precatorius

Organism

Organism	UniProt	Comment	Textmining
Abrus precatorius	-	harvested in Ngaoundere, Adamawa, region in Northern Cameroon	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme enriched fraction from powdered stems of Abrus precatorius using a three-phase partitioning method and a Doehlert design with 3 variables (ratio of crude extract/t-butanol, the ammonium sulfate saturation and pH). Data fitting in a second-order polynomial model and parameters optimization to enrich beta-amylase. Optimal conditions are: a ratio of crude extract/tert-butanol of 0.87 v/v, saturation in ammonium sulfate of 49.46% w/v, and a pH of 5.2. An activity recovery of 156.2% and a purification factor of 10.17 are found. Next steps are dialysis and lyophilisation	Abrus precatorius

Purification (Comment)

Organism

native enzyme enriched fraction from powdered stems of Abrus precatorius using a three-phase partitioning method and a Doehlert design with 3 variables (ratio of crude extract/t-butanol, the ammonium sulfate saturation and pH). Data fitting in a second-order polynomial model and parameters optimization to enrich beta-amylase. Optimal conditions are: a ratio of crude extract/tert-butanol of 0.87 v/v, saturation in ammonium sulfate of 49.46% w/v, and a pH of 5.2. An activity recovery of 156.2% and a purification factor of 10.17 are found. Next steps are dialysis and lyophilisation

Abrus precatorius

Source Tissue

Source Tissue	Comment	Organism	Textmining
stem	-	Abrus precatorius	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.79	-	crude enzyme extract, pH 6.0, 50°C	Abrus precatorius
19.99	-	TPP-interfacial precipitate enzyme after dialysis and lyophilisation, pH 6.0, 50°C	Abrus precatorius
24.59	-	TPP-interfacial precipitate enzyme after dialysis, pH 6.0, 50°C	Abrus precatorius
38.24	-	TPP-interfacial precipitate enzyme, pH 6.0, 50°C	Abrus precatorius

Subunits

Subunits	Comment	Organism
?	x * 60100, SDS-PAGE	Abrus precatorius

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	native enzyme	Abrus precatorius

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
65	-	purified native enzyme, pH 6.0-6.5, stable up to	Abrus precatorius

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	6.5	native enzyme	Abrus precatorius