Application | Comment | Organism |
---|---|---|
industry | the recombinant purified alpha-amylase enzyme from Thermotoga petrophila can be a potential candidate for use in many industrial processes; particularly, it can be helpful in starch saccharification due to the adaptive capability of the alpha-amylase to withstand unreceptive conditions used in industrial processes | Thermotoga petrophila |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3), pET 21a (+) vector | Thermotoga petrophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 4%, 1h, activity is decreased to 53% | Thermotoga petrophila | |
acetone | 40%, relative activity is decreased to 87% | Thermotoga petrophila | |
Co2+ | 1 mM, 37% loss of activity | Thermotoga petrophila | |
EDTA | 1 mM, 42% loss of activity. 5-10 mM, complete loss of activity | Thermotoga petrophila | |
Isopropanol | 40%, relative activity is decreased to 80% | Thermotoga petrophila | |
K+ | 1 mM, 1% loss of activity | Thermotoga petrophila | |
Mg2+ | 1 mM, 14% loss of activity | Thermotoga petrophila | |
additional information | no considerable effect of organic solvents (ethanol, methanol, isopropanol, acetone and n-butanol) is observed on enzyme activity | Thermotoga petrophila | |
n-butanol | 40%, relative activity is decreased to 85% | Thermotoga petrophila | |
Ni2+ | 1 mM, 28% loss of activity | Thermotoga petrophila | |
SDS | 4%, 1 h, complete loss of activity | Thermotoga petrophila | |
Tween 20 | 4%, 1h, activity is decreased to 71% | Thermotoga petrophila | |
Tween 80 | 4%, 1h, activity is decreased to 61% | Thermotoga petrophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activity is increased to 150 % in the presence of calcium chloride | Thermotoga petrophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
SDS-PAGE | Thermotoga petrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga petrophila | A0A0U4KTC9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga petrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopektin + H2O | - |
Thermotoga petrophila | ? | - |
? | |
dextrin + H2O | - |
Thermotoga petrophila | ? | - |
? | |
soluble starch + H2O | maximum activity with 1.2 % soluble starch. Less activity is observed with starch from corn, rice, glycogen | Thermotoga petrophila | maltose + ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermotoga petrophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
2 h, purified enzyme retains 100 % relative activity. Ater 4 h of incubation, the relative activity of the enzyme is 80% | Thermotoga petrophila |
60 | - |
2 h, purified enzyme retains 100 % relative activity. Ater 4 h of incubation, the relative activity of the enzyme is 80% | Thermotoga petrophila |
70 | - |
2 h, purified enzyme retains 100 % relative activity. Ater 4 h of incubation, the relative activity of the enzyme is 72% | Thermotoga petrophila |
80 | - |
2 h, purified enzyme retains 100 % relative activity. Ater 4 h of incubation, the relative activity of the enzyme is 63% | Thermotoga petrophila |
90 | - |
2 h, 15% loss of activity. Ater 4 h of incubation, the relative activity of the enzyme is 54% | Thermotoga petrophila |
100 | - |
2 h, 22% loss of activity. Ater 4 h of incubation, the relative activity of the enzyme is 45% | Thermotoga petrophila |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
after 1 h of incubation, the relative activity of the enzyme is 20% | Thermotoga petrophila |
5 | - |
after 1 h of incubation, the relative activity of the enzyme is 30% | Thermotoga petrophila |
6 | - |
the enzyme exhibits 80% relative activity after 4 h | Thermotoga petrophila |
7 | - |
maximum relative activity (100%) is obtained after 4 h at pH 7.0 | Thermotoga petrophila |
8 | - |
the enzyme exhibits 70% relative activity after 3 h | Thermotoga petrophila |
9 | - |
activity is decreased to 30% after 1 h of incubation | Thermotoga petrophila |