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Literature summary for 3.2.1.2 extracted from

  • Sparla, F.; Costa, A.; Lo Schiavo, F.; Pupillo, P.; Trost, P.
    Redox regulation of a novel plastid-targeted beta-amylase of Arabidopsis (2006), Plant Physiol., 141, 840-850.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.2

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme requires reducing conditions for full activity Arabidopsis thaliana
reduced DTT strong activation of isozyme TR-BAMY Arabidopsis thaliana
thioredoxin thioredocins f1, m1, and y1, the isozyme TR-BAMY is positively thioredoxin-regulated Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
isozyme CT-BAMY, DNA and amino acid sequence determination and analysis Arabidopsis thaliana
isozyme TR-BAMY, DNA and amino acid sequence determination and analysis, functional expression of mature isozyme TR-BAMY and of mutant enzymes in Escherichia coli strains HB101 and BL21(DE3), transient expression of GFP-tagged isozyme TR-BAMY in Nicotiana tabacum protoplasts with exclusive association to the chloroplasts Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C148S site-directed mutagenesis, the mutant shows a redox sensitivity similar to the wild-type enzyme Arabidopsis thaliana
C206S site-directed mutagenesis, the mutant shows a redox sensitivity similar to the wild-type enzyme Arabidopsis thaliana
C261S site-directed mutagenesis, the mutant shows a redox sensitivity similar to the wild-type enzyme Arabidopsis thaliana
C32S site-directed mutagenesis, the mutant shows 70% impaired redox sensitivity compared to the wild-type enzyme Arabidopsis thaliana
C399S site-directed mutagenesis, the mutant shows a redox sensitivity similar to the wild-type enzyme Arabidopsis thaliana
C413S site-directed mutagenesis, the mutant shows a redox sensitivity similar to the wild-type enzyme Arabidopsis thaliana
C470S site-directed mutagenesis, the mutant shows 70% impaired redox sensitivity compared to the wild-type enzyme Arabidopsis thaliana
C506S site-directed mutagenesis, the mutant shows a redox sensitivity similar to the wild-type enzyme Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
additional information the 41 amino acid transit peptide inhibits isozyme TR-BAMY Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast plastid-targeted isozyme CT-BAMY, contains a plastid transit peptide Arabidopsis thaliana 9507
-
chloroplast plastid-targeted isozyme TR-BAMY, contains a plastid transit peptide of 41 amino acids Arabidopsis thaliana 9507
-
plastid plastid-targeted isozyme TR-BAMY, contains a plastid transit peptide of 41 amino acids Arabidopsis thaliana 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 recombinant mature isozyme TR-BAMY, gel filtration Arabidopsis thaliana
64000
-
 recombinant precursor isozyme TR-BAMY, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana the isozyme TR-BAMY is redox- and thioredoxin-regulated involving residues C470 and C32 forming a disulfide bridge, the precursor enzyme conatining the transit pepetide is inactive ?
-
 ?
starch + H2O Arabidopsis thaliana
-
 maltose + ?
-
 ?
starch + H2O Arabidopsis thaliana the isozyme is involved in leaf starch degradation maltose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O23553 gene At4g170990, isozyme CT-BAMY; contains six extrachlorplastic isozymes, and three plastid-targeted isozymes
-
Arabidopsis thaliana Q9LIR6 gene At3g23920/F14O13_11, isozyme TR-BAMY; contains six extrachloroplastic isozymes, and three plastid-targeted isozymes
-

Purification (Commentary)

Purification (Comment) Organism
native isozyme partially by chloroplast purification Arabidopsis thaliana
native isozyme partially by chloroplast purification, recombinant mature isozyme TR-BAMY and recombinant mutant enzymes from Escherichia coli Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower isozyme TR-BAMY Arabidopsis thaliana
-
leaf isozyme TR-BAMY Arabidopsis thaliana
-
pollen isozyme TR-BAMY Arabidopsis thaliana
-
root isozyme TR-BAMY Arabidopsis thaliana
-
seed isozyme TR-BAMY Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl maltopentaose + H2O
-
 Arabidopsis thaliana 4-nitrophenol + maltopentaose
-
 ?
additional information the isozyme TR-BAMY is redox- and thioredoxin-regulated involving residues C470 and C32 forming a disulfide bridge, the precursor enzyme conatining the transit pepetide is inactive Arabidopsis thaliana ?
-
 ?
starch + H2O
-
 Arabidopsis thaliana maltose + ?
-
 ?
starch + H2O soluble starch Arabidopsis thaliana maltose + ?
-
 ?
starch + H2O the isozyme is involved in leaf starch degradation Arabidopsis thaliana maltose + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 60000, recombinant mature isozyme TR-BAMY, SDS-PAGE, 1 * 64000, recombinant precursor isozyme TR-BAMY, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
CT-BMY
-
 Arabidopsis thaliana
TR-BAMY
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 the isozyme TR-BAMY shifts between an active and an inactive form dependent on the pH Arabidopsis thaliana
6 8
-
 Arabidopsis thaliana