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Literature summary for 3.2.1.2 extracted from
- Purification and characterization of extracellular beta-amylase of Bacillus megaterium B6 (2000), Acta Microbiol. Immunol. Hung., 47, 29-40.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, activates | Priestia megaterium |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | 1 mM, 94% inhibition | Priestia megaterium | |
| Al2(SO4)3 | 1 mM, 57% inhibition | Priestia megaterium | |
| Bi(NO3)3 | 1 mM, 54% inhibition | Priestia megaterium | |
| CuCl2 | 1 mM, 93% inhibition | Priestia megaterium | |
| FeCl3 | 1 mM, 16% inhibition | Priestia megaterium | |
| HgCl2 | 1 mM, 93% inhibition | Priestia megaterium | |
| N-ethylmaleimide | remarkably reduces activity | Priestia megaterium | |
| p-chloromercuribenzoate | 1 mM, 97% inhibition, exogenous thiols like dithiothreitol, 2-mercaptoethanol or cysteine HCl reactivate | Priestia megaterium | |
| sodium deoxycholate | 0.001%, 15% inhibition | Priestia megaterium | |
| Sodium dodecyl sulfate | 0.001%, 57% inhibition | Priestia megaterium | |
| Tween 20 | 0.001%, 16% inhibition | Priestia megaterium | |
| Tween 40 | 0.001%, 15% inhibition | Priestia megaterium | |
| ZnSO4 | 1 mM, 54% inhibition | Priestia megaterium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km for starch is 4.34 mg/ml at 60°C | Priestia megaterium |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Priestia megaterium | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| BaCl2 | 1 mM, activates | Priestia megaterium | |
| CaCl2 | 1 mM, activates | Priestia megaterium | |
| CoCl2 | 1 mM, activates | Priestia megaterium | |
| MnCl2 | 1 mM, activates | Priestia megaterium | |
| SrCl2 | 1 mM, activates | Priestia megaterium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 102000 | - |
1 * 102000, SDS-PAGE | Priestia megaterium |
| 105000 | - |
gel filtration | Priestia megaterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | - |
- |
- |
| Priestia megaterium B6 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 4100fold | Priestia megaterium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Priestia megaterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | 88% of the activity with starch | Priestia megaterium | ? | - |
? | |
| amylopectin + H2O | 88% of the activity with starch | Priestia megaterium B6 | ? | - |
? | |
| amylose + H2O | 79% of the activity with starch | Priestia megaterium | ? | - |
? | |
| amylose + H2O | 79% of the activity with starch | Priestia megaterium B6 | ? | - |
? | |
| dextrin + H2O | 13% of the activity with starch | Priestia megaterium | ? | - |
? | |
| dextrin + H2O | 13% of the activity with starch | Priestia megaterium B6 | ? | - |
? | |
| additional information | not: xylan, pullulan, cellulose, carboxymethyl cellulose | Priestia megaterium | ? | - |
? | |
| additional information | not: xylan, pullulan, cellulose, carboxymethyl cellulose | Priestia megaterium B6 | ? | - |
? | |
| starch + H2O | best substrate, pure and low quality starches, maize starch, tapioca starch | Priestia megaterium | beta-maltose | maltose is the major end product, traces of maltooligosaccharides, no glucose as product | ? | |
| starch + H2O | best substrate, pure and low quality starches, maize starch, tapioca starch | Priestia megaterium B6 | beta-maltose | maltose is the major end product, traces of maltooligosaccharides, no glucose as product | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 102000, SDS-PAGE | Priestia megaterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
temperature optimum, but enzyme loses activity when exposed to 60°C, irreversible thermodenaturation | Priestia megaterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
temperature optimum, but enzyme loses activity when exposed to 60°C, irreversible thermodenaturation, thermodenaturation kinetics, Mn2+ and exogenous thiols like dithiothreitol, 2-mercaptoethanol or cysteine HCl play a remarkable role in reactivation of thermally denatured enzyme | Priestia megaterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
- |
Priestia megaterium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | 90% stability between pH 6.5 and 7.5 | Priestia megaterium |
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