Cloned (Comment) | Organism |
---|---|
expression of a sevenfold mutant beta-amylase, M185L/S295A/I297V/S350P/S351P/Q352D/A376S | Hordeum vulgare |
Protein Variants | Comment | Organism |
---|---|---|
M185L/S295A/I297V/S350P/S351P/Q352D/A376S | the mutant enzyme acquires enhanced thermostability, but its function as beta-amylase is unchanged. The mutant is stable at pH-values up to 12.5, while the original recombinant enzyme is unstable at pH-values above pH 9.5 | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, almost complete inhibition of mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S | Hordeum vulgare | |
Cd2+ | 1 mM, almost complete inhibition of barley enzyme and recombinant enzyme, less inhibitory towards mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S | Hordeum vulgare | |
Cu2+ | 1 mM, almost complete inhibition of mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S | Hordeum vulgare | |
Hg2+ | 1 mM, almost complete inhibition of mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S | Hordeum vulgare | |
PCMB | - |
Glycine max | |
PCMB | 0.1 mM, complete inhibition of mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S | Hordeum vulgare | |
Zn2+ | 1 mM, almost complete inhibition of barley enzyme and recombinant enzyme, less inhibitory towards mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.491 | - |
amylose | DP = 17, mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S | Hordeum vulgare | |
1.83 | - |
maltoheptaose | mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S | Hordeum vulgare | |
2 | - |
maltohexaose | mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S | Hordeum vulgare | |
2.83 | - |
maltopentaose | mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S | Hordeum vulgare | |
4.17 | - |
maltotetraose | mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S, SDS-PAGE | Hordeum vulgare |
59144 | - |
x * 59144, mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S, calculation from amino acid sequence | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Hordeum vulgare | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Hordeum vulgare | beta-maltose + ? | - |
? | |
amylopectin + H2O | - |
Glycine max | beta-maltose + ? | - |
? | |
amylose + H2O | DP = 17 | Hordeum vulgare | beta-maltose | - |
? | |
glycogen + H2O | from oyster | Hordeum vulgare | beta-maltose | - |
? | |
maltoheptaose + H2O | - |
Hordeum vulgare | ? | - |
? | |
maltohexaose + H2O | - |
Hordeum vulgare | ? | - |
? | |
maltopentaose + H2O | - |
Hordeum vulgare | ? | - |
? | |
maltotetraose + H2O | - |
Hordeum vulgare | ? | - |
? | |
starch + H2O | soluble | Hordeum vulgare | maltose + ? | - |
? | |
starch + H2O | soluble | Glycine max | maltose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Glycine max |
? | x * 59144, mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S, calculation from amino acid sequence | Hordeum vulgare |
? | x * 56000, mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S, SDS-PAGE | Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
original and recombinant enzyme | Hordeum vulgare |
60 | - |
- |
Glycine max |
65 | - |
mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S | Hordeum vulgare |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
30 min, 50% loss of activity | Hordeum vulgare |
57 | - |
30 min, recombinant enzyme, 50% loss of activity | Hordeum vulgare |
63 | - |
30 min, 50% loss of activity | Glycine max |
69 | - |
30 min, mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S, 50% inactivation | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Hordeum vulgare |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
unstable above, original recombinant enzyme | Hordeum vulgare |
12.5 | - |
mutant enzyme M185L/S295A/I297V/S350P/S351P/Q352D/A376S is stable up to | Hordeum vulgare |