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Literature summary for 3.2.1.2 extracted from
- Detection, purification and partial characterization of beta-amylase from trophozoites of Entamoeba invadens (1996), Mol. Biochem. Parasitol., 83, 175-181.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Entamoeba invadens |  |
| PCMB | - |
Entamoeba invadens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activates | Entamoeba invadens | |
| cysteine | activates | Entamoeba invadens | |
| dithiothreitol | activates | Entamoeba invadens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
gel filtration | Entamoeba invadens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba invadens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Entamoeba invadens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| trophozoite | - |
Entamoeba invadens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 65.1 | - |
- |
Entamoeba invadens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for 3 weeks | Entamoeba invadens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-maltoheptaoside + H2O | - |
Entamoeba invadens | ? | - |
? | |
| amylopectin + H2O | - |
Entamoeba invadens | beta-maltose + ? | and limit dextrin | ? | |
| amylose + H2O | - |
Entamoeba invadens | beta-maltose | - |
? | |
| glycogen + H2O | - |
Entamoeba invadens | beta-maltose | and limit dextrin | ? | |
| additional information | no activity with pullulan | Entamoeba invadens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 45000, SDS-PAGE | Entamoeba invadens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
degradation of glycogen | Entamoeba invadens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 29 | 41 | 75% of maximal activity at 29°C and at 41°C | Entamoeba invadens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
degradation of glycogen | Entamoeba invadens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7.2 | 50% of maximal activity at pH 3.0 and pH 7.2, degradation of glycogen | Entamoeba invadens |
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