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Literature summary for 3.2.1.2 extracted from
- Purification and characterization of pea epicotyl beta-amylase (1990), Plant Physiol., 92, 615-621.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-cyclohexaamylose | - |
Pisum sativum |  |
| Cu2+ | CuCl2 | Pisum sativum | |
| Hg2+ | - |
Pisum sativum | |
| iodoacetamide | - |
Pisum sativum | |
| maltose | competitive | Pisum sativum | |
| NEM | - |
Pisum sativum | |
| p-chloromercuriphenyl sulfonic acid | - |
Pisum sativum | |
| Schardinger maltodextrins | partial | Pisum sativum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km: 1.67 mg/ml for soluble starch | Pisum sativum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
gel filtration | Pisum sativum |
| 57000 | - |
1 * 57000, SDS-PAGE | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-2-alpha-D-glucopyranose + alpha-D-glucopyranosyl-(1-4)-beta-D-glucopyranose | multichain type mechanism | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | epicotyl of etiolated germinating seedlings | Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1030 | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Pisum sativum | beta-maltose + ? | - |
? | |
| amylose + H2O | - |
Pisum sativum | beta-maltose | - |
? | |
| glycogen + H2O | - |
Pisum sativum | beta-maltose | - |
? | |
| maltoheptaose + H2O | - |
Pisum sativum | ? | - |
? | |
| additional information | moderately branched glucans are better substrates than less branched or non-branched or highly branched glucans | Pisum sativum | ? | - |
? | |
| additional information | no hydrolysis of beta-limit dextrin | Pisum sativum | ? | - |
? | |
| starch + H2O | grains | Pisum sativum | maltose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 57000, SDS-PAGE | Pisum sativum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
thermal denaturation above | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pisum sativum |
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Release 2023.1 (January 2023)
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