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Literature summary for 3.2.1.2 extracted from

  • Takasaki, Y.
    Purification and enzymatic properties of beta-amylase and pullulanase from Bacillus cereus var. mycoides (1976), Agric. Biol. Chem., 40, 1523-1530.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag2+
-
Bacillus cereus
Cu2+
-
Bacillus cereus
Fe2+
-
Bacillus cereus
Hg2+ HgCl2 Bacillus cereus
PCMB cysteine reactivates Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
gel filtration Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
var. mycoides
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Bacillus cereus beta-maltose + ?
-
?
amylose + H2O
-
Bacillus cereus beta-maltose
-
?
glycogen + H2O
-
Bacillus cereus beta-maltose
-
?
starch + H2O
-
Bacillus cereus maltose + ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus cereus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
28 61 28°C: about 40% of maximal activity, 61°C: about 30% of maximal activity Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, stable below Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bacillus cereus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 pH 4.5: about 40% of maximal activity, pH 8.5: about 60% of maximal activity Bacillus cereus