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Literature summary for 3.2.1.195 extracted from
- Characterization of a novel arabinose-tolerant alpha-L-arabinofuranosidase with high ginsenoside Rc to ginsenoside Rd bioconversion productivity (2016), J. Appl. Microbiol., 120, 647-660.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | in a reaction at 85°C and pH 5.0, 25 g/l of ginsenoside Rc is transformed into 21.8 g/l of ginsenoside Rd within 60 min, with a corresponding molar conversion of 99.4% and a high ginsenoside Rd productivity of 21800 mg/l/h | Pseudothermotoga thermarum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudothermotoga thermarum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | 1 mM, no residual activtiy | Pseudothermotoga thermarum |  |
| additional information | not inhibitory at 1 mM: EDTA | Pseudothermotoga thermarum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE | Pseudothermotoga thermarum |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| DMSO | 10% v/v, 69% residual activity | Pseudothermotoga thermarum |
| Ethanol | 10% v/v, 71% residual activity | Pseudothermotoga thermarum |
| Methanol | 10% v/v, 56% residual activity | Pseudothermotoga thermarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudothermotoga thermarum | F7YTI3 | - |
- |
| Pseudothermotoga thermarum DSM 5069 | F7YTI3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ginsenoside Rc + H2O | - |
Pseudothermotoga thermarum | ginsenoside Rd + L-arabinofuranose | - |
? | |
| ginsenoside Rc + H2O | - |
Pseudothermotoga thermarum DSM 5069 | ginsenoside Rd + L-arabinofuranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Pseudothermotoga thermarum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Theth_1120 | - |
Pseudothermotoga thermarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Pseudothermotoga thermarum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 100 | more than 50% of maximum activtiy | Pseudothermotoga thermarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Pseudothermotoga thermarum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7.5 | stable | Pseudothermotoga thermarum |
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Release 2023.1 (January 2023)
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