Cloned (Comment) | Organism |
---|---|
expressed in MDCK cells | influenza A virus H1N1 |
Protein Variants | Comment | Organism |
---|---|---|
D199E | the mutant shows increased enzyme activity compared to the wild type enzyme | influenza A virus H1N1 |
E119D | the mutation confers reduced inhibition by oseltamivir (a 95-fold increase in the 50% inhibitory concentration) and highly reduced inhibition by zanamivir, peramivir. and laninamivir (2660fold, 460fold, and 651fold increases in the 50% inhibitory concentration, respectively) | influenza A virus H1N1 |
E119G | the mutation confers mainly reduced to highly reduced inhibition by zanamivir, peramivir, and laninamivir, but remains susceptible to oseltamivir | influenza A virus H1N1 |
I321V | the mutant shows normal inhibition by zanamivir, peramivir, laninamivir and oseltamivir compared to the wild type enzyme | influenza A virus H1N1 |
K432E | the mutant shows normal inhibition by zanamivir, peramivir, laninamivir and oseltamivir compared to the wild type enzyme | influenza A virus H1N1 |
N200S | the mutant shows normal inhibition by zanamivir, peramivir, laninamivir and oseltamivir compared to the wild type enzyme | influenza A virus H1N1 |
N248D | the mutant shows normal inhibition by zanamivir, peramivir, laninamivir and oseltamivir compared to the wild type enzyme | influenza A virus H1N1 |
N270K | the mutant shows normal inhibition by zanamivir, peramivir, laninamivir and oseltamivir compared to the wild type enzyme | influenza A virus H1N1 |
N369K | the mutant shows normal inhibition by zanamivir, peramivir, laninamivir and oseltamivir compared to the wild type enzyme | influenza A virus H1N1 |
N386K | the mutant shows normal inhibition by zanamivir, peramivir, laninamivir and oseltamivir compared to the wild type enzyme | influenza A virus H1N1 |
P458T | the mutant with decreased activity exhibits highly reduced inhibition by zanamivir, peramivir, laninamivir and oseltamivir compared to the wild type enzyme | influenza A virus H1N1 |
Q136K | the mutation confers mainly reduced to highly reduced inhibition by zanamivir, peramivir, and laninamivir, but remains susceptible to oseltamivir | influenza A virus H1N1 |
R152K | the mutant remains susceptible to peramivir, but confers reduced inhibition by zanamivir, oseltamivir, and laninamivir | influenza A virus H1N1 |
V264I | the mutant shows normal inhibition by zanamivir, peramivir, laninamivir and oseltamivir compared to the wild type enzyme | influenza A virus H1N1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
laninamivir | - |
influenza A virus H1N1 | |
oseltamivir | - |
influenza A virus H1N1 | |
peramivir | - |
influenza A virus H1N1 | |
zanamivir | - |
influenza A virus H1N1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid | wild type enzyme, at 37°C, pH not specified in the publication | influenza A virus H1N1 | |
0.2595 | - |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid | mutant enzyme D199E, at 37°C, pH not specified in the publication | influenza A virus H1N1 | |
0.6458 | - |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid | mutant enzyme P458T, at 37°C, pH not specified in the publication | influenza A virus H1N1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
influenza A virus H1N1 | - |
A/California/04/2009 | - |
influenza A virus H1N1 pdm09 | - |
A/California/04/2009 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O | - |
influenza A virus H1N1 | 4-methylumbelliferone + alpha-D-N-acetylneuraminic acid | - |
? | |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O | - |
influenza A virus H1N1 pdm09 | 4-methylumbelliferone + alpha-D-N-acetylneuraminic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neuraminidase | - |
influenza A virus H1N1 |