Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Trypsin | isoform NanI activity is enhanced by trypsin pretreatment and then only for substrates with an alpha-2,3- or alpha-2,6-sialic acid linkage | Clostridium perfringens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 90% residual activity at 10 mM | Clostridium perfringens | |
Fe3+ | 56% residual activity at 10 mM | Clostridium perfringens | |
additional information | not inhibited by EDTA | Clostridium perfringens | |
N-acetyl-2,3-dehydro-2-deoxyneuraminic acid | - |
Clostridium perfringens | |
p-chloromercuribenzoate | 18% residual activity at 10 mM | Clostridium perfringens | |
siastatin B | - |
Clostridium perfringens | |
Zn2+ | 61% residual activity at 10 mM | Clostridium perfringens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 110% activity at 10 mM | Clostridium perfringens | |
Mg2+ | 115% activity at 10 mM | Clostridium perfringens | |
Mn2+ | 110% activity at 10 mM | Clostridium perfringens | |
additional information | not influenced by Fe2+ and Ni2+ | Clostridium perfringens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, isoform NanH, SDS-PAGE | Clostridium perfringens |
77000 | - |
x * 77000, isoform NanI, SDS-PAGE | Clostridium perfringens |
129000 | - |
x * 129000, isoform NanJ, SDS-PAGE | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | - |
type D | - |
Clostridium perfringens CN3718 | - |
type D | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Clostridium perfringens | 5-bromo-4-chloro-3-hydroxyindole + N-acetylneuraminic acid | - |
? | |
5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Clostridium perfringens CN3718 | 5-bromo-4-chloro-3-hydroxyindole + N-acetylneuraminic acid | - |
? | |
additional information | siladiases possess activity against an alpha-2,3-specific linkage, an alpha-2,6-specific linkage, and an alpha-2,8-specific linkage. The observed activity is in the following order: alpha-2,3 > alpha-2,6 > alpha-2,8 sialic acid linkages | Clostridium perfringens | ? | - |
? | |
additional information | siladiases possess activity against an alpha-2,3-specific linkage, an alpha-2,6-specific linkage, and an alpha-2,8-specific linkage. The observed activity is in the following order: alpha-2,3 > alpha-2,6 > alpha-2,8 sialic acid linkages | Clostridium perfringens CN3718 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 129000, isoform NanJ, SDS-PAGE | Clostridium perfringens |
? | x * 43000, isoform NanH, SDS-PAGE | Clostridium perfringens |
? | x * 77000, isoform NanI, SDS-PAGE | Clostridium perfringens |
Synonyms | Comment | Organism |
---|---|---|
NanH | isoform | Clostridium perfringens |
NanI | isoform | Clostridium perfringens |
NanJ | isoform | Clostridium perfringens |
sialidase | - |
Clostridium perfringens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
isoform NanJ | Clostridium perfringens |
43 | - |
isoform NanH | Clostridium perfringens |
48 | - |
isoform NanI sialidase activity steadily increases with temperature until 48°C | Clostridium perfringens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the NanI sialidase shows no loss of activity after a 10 min incubation at 50°C in Tris-HCl buffer, pH 7.2 | Clostridium perfringens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Clostridium perfringens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | - |
at pH 9.0, the activity of all three sialidase isoforms has decreased by about 50% | Clostridium perfringens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0189 | - |
at pH 7.2 and 37°C | Clostridium perfringens | N-acetyl-2,3-dehydro-2-deoxyneuraminic acid | |
0.0422 | - |
at pH 7.2 and 37°C | Clostridium perfringens | siastatin B |