Cloned (Comment) | Organism |
---|---|
expression of His-tagged viral neuraminidase in wild-type Pichia pastoris strain GS115, an alpha-1,6-mannosyltransferase (och1)-defective Pichia pastoris mutant strain, and in Escherichia coli, resulting in a hyper-glycosylated, a low-glycosylated, and a non-glycosylated enzyme, respectively | influenza A virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 59000-97000, hyperglycosylated recombinant enzyme, SDS-PAGE, x * 66000, low-glycosylated recombinant enzyme, SDS-PAGE, x * 45000, non-glycosylated recombinant enzyme, SDS-PAGE | influenza A virus |
66000 | - |
x * 59000-97000, hyperglycosylated recombinant enzyme, SDS-PAGE, x * 66000, low-glycosylated recombinant enzyme, SDS-PAGE, x * 45000, non-glycosylated recombinant enzyme, SDS-PAGE | influenza A virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
influenza A virus | Q7TG96 | - |
- |
influenza A virus newCaledonia/20/99(H1N1) | Q7TG96 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged viral neuraminidase by nickel affinity chromatography, desalting gel filtration, and cation exchange chromatography | influenza A virus |
Subunits | Comment | Organism |
---|---|---|
? | x * 59000-97000, hyperglycosylated recombinant enzyme, SDS-PAGE, x * 66000, low-glycosylated recombinant enzyme, SDS-PAGE, x * 45000, non-glycosylated recombinant enzyme, SDS-PAGE | influenza A virus |
Synonyms | Comment | Organism |
---|---|---|
neuraminidase | - |
influenza A virus |
General Information | Comment | Organism |
---|---|---|
additional information | low-glycosylation in och1-defective Pichia pastoris enhances the immunogenicity of recombinant neuraminidase and elicits similar antibody titers with less antigen when compared with hyper- and non-glycosylated neuraminidases after vaccination of mice | influenza A virus |